| 利用菌落免疫荧光法鉴定对多种支原体起反应的单克隆抗体 |
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| 引用本文： | 杨保安,孟玲珍,辛颜彬.利用菌落免疫荧光法鉴定对多种支原体起反应的单克隆抗体[J].军事医学科学院院刊,1991(3). |
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| 作者姓名： | 杨保安 孟玲珍 辛颜彬 |
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| 作者单位： | 军事医学科学院微生物学流行病学研究所，军事医学科学院微生物学流行病学研究所，军事医学科学院微生物学流行病学研究所 |
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| 摘 要： | 我们用菌落免疫荧光染色技术对4株(3D_1、3A_(11)、3F_5、2E_5)抗精氨酸支原体(MycoPlasma arginini)McAb进行了鉴定,在热水固定的菌落上3D_1、3A_(11)不仅与精氨酸支原体出现了阳性反应,而且与口腔支原体(M.orale)、猪鼻支原体(M.hyorhinis)、莱氏无胆甾原体(AcholePlasma laidlawii)、羊无乳支原体(M.agalactia)PG_2和牛丝状支原体丝状亚种(M.mycoides subsp. mycoides)PG_1等均有交叉反应;在未固定的菌落上用表面免疫荧光检测时, McAb却表现了与PcAb不同的特性。同时我们还做了阻断试验,进一步证明了我们制备的McAb的支原体特异性。
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| 关 键 词： | 支原体属 单克隆抗体 菌落染色 |
IDENTIFICATION OF MONOCLONAL ANTIBODIES WITH UNIVERSAL REACTION TO MYCOPLASMAS BY COLONY IFA TEST |
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| Yang Baoan Meng Lingzen Xin Yanbin.IDENTIFICATION OF MONOCLONAL ANTIBODIES WITH UNIVERSAL REACTION TO MYCOPLASMAS BY COLONY IFA TEST[J].Bulletin of the Academy of Military Medical Sciences,1991(3). |
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| Authors: | Yang Baoan Meng Lingzen Xin Yanbin |
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| Abstract: | Four monoclonal antibodies (3D_1, 3A_(11), 3F_5 and 2E_5) against MyaoPlasma arginini were identified with colony IFA technique. At the colonies fixed by the hot water, 3D_1 and 3A_(11) not only revealed positive reaction with M. arginini, but also crossly reacted with M. orale, M. hyorhinis, A. laidlawii, M. agalactia (PG2), and M. mycoides subsp, myeoides (PG1). But at the unfixed colonies, the monoclonal antibodies had different characteristics from polyclonal antibodies. The results obtained by blocking test demonstrated the specificity of our prepared McAb against mycoplasma. |
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| Keywords: | mycoplasma monoclonal antibodies colony IFA technique |
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