胰腺癌组织中端粒酶活性检测及基因表达

目的　探讨胰腺癌组织端粒酶活性和端粒酶基因表达及两者的相关性 ,及其与癌生物学行为的关系 ,以评价端粒酶活性和端粒酶基因表达检测对胰腺癌的诊断价值。方法　采用TRAP -PCR -ELISA方法和原位杂交方法 ,检测了 2 3例胰腺癌患者端粒酶活性和基因表达情况。结果　 2 3例胰腺癌组织端粒酶阳性率为 87.0 % ,10例癌周组织阳性率为 10 .0 % ,两者有显著性差异 (P <0 .0 5 )。 2 3例胰腺癌组织中 19例 (82 .6% )hTR表达阳性 ,10例癌周组织中 1例 (10 .0 % )hTR表达阳性。胰腺癌组织中hTR表达阳性率与癌周组织比较 ,有显著性差异 (P <0 .0 5 )。端粒酶活性及其基因表达与胰腺癌组织学分级、临床分期及伴随局部淋巴结转移无明显相关性 (P >0 .0 5 )。结果还显示端粒酶活性与hTR基因表达相关 (P <0 .0 5 )。结论　端粒酶可能是胰腺癌发生的早期事件 ,端粒酶活性及端粒酶基因表达的检测对胰腺癌具有一定的诊断价值

Telomerase Activity and Telomerase Gene Expression in Pancreatic Cancer

Objective To investigate telomerase activity and telomerase gene expression in pancreatic cancer and to compare the telomerase activity and telomerase gene expression,in order to evaluate the role of detection of telomerase activity and gene in tumor diagnosis.Methods With TRAP PCR ELISA and in situ hybridization,telomerase activity and telomerase hTR gene expression were studied in 23 cases of pancreatic cancer tisssues,10 cases of adjacent tissues,the results were statistically analyzed.Results The positive rate of telomerase was 87.0%(20/23) of pancreatic cancer tissues and 10.0%(1/10) of adjacent tissues,respectively.hTR was detected in 82.6%(19/23) of pancreatic cancer.While in the adjacent tissues,the positive rates were only 10.0%(1/10).The positive detection of hTR expression in cancer group were significantly different from those in the adjacent tissues( P <0.05).Telomerase activity and telomerase gene expression levels were not related to the degrees of cell differentiation,clinical stage and lymph node metastasis of pancreatic cancer( P >0.05).Besides,the telomerase activity and expression of telomerase genes were noticed to be also highly correlated( P <0.05).Conclusion Telomerase may be an earlier event of malignant progression in pancreatic cancer.Detection of telomerase activity and expression of telomerase gene is useful in the diagnosis of pancreatic cancer.