| 双自杀基因重组腺病毒对肺癌细胞的杀伤作用 |
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| 引用本文： | 黄畦,陈大瑜,付向宁,祖育昆. 双自杀基因重组腺病毒对肺癌细胞的杀伤作用[J]. 中华实验外科杂志, 2006, 23(4): 579-580 |
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| 作者姓名： | 黄畦 陈大瑜 付向宁 祖育昆 |
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| 作者单位： | 430030,武汉,华中科技大学同济医学院同济医院心胸外科 |
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| 摘 要： | 目的构建含CD／UPRT双自杀基因的重组腺病毒Ad—CD／UPRT，观察其对人肺腺癌细胞的杀伤作用。方法用0～100感染复数的重组腺病毒转染肺腺癌A549细胞后加入（0～1000）mg／L的5-氟胞嘧啶（5-FC），MTT法检测杀伤效率；将转基因细胞与未转基因细胞按不同比例混合，观察旁观者效应。结果Ad-CD／UPRT转染A549后对细胞生长有显著的抑制作用，细胞杀伤效率随腺病毒滴度及5-FC浓度的增加而增强；转基因细胞占混合细胞10％以上，即可观察到明显的旁观者效应。结论Ad—CD／UPRT联合5-FC对肺腺癌A549细胞有显著杀伤作用。
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| 关 键 词： | 肺癌 腺病毒 基因治疗 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005-06-28 |
Killing effects of recombinant adenovirus vector containing double suicide gene on pulmonary carcinoma cells |
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| HUNAG Qi, CHEN Da-yu, FU Xiang-ning,et al.. Killing effects of recombinant adenovirus vector containing double suicide gene on pulmonary carcinoma cells[J]. Chinese Journal of Experimental Surgery, 2006, 23(4): 579-580 |
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| Authors: | HUNAG Qi CHEN Da-yu FU Xiang-ning et al. |
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| Affiliation: | Department of Cardiothoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective To construct a recombinant adenovirus vector containing CD/UPRT double suicide gene and to evaluate the specific killing effects on pulmonary carcinoma in vitro. Methods A549 cells were infected with 0, 1, 10,100 MOI of Ad-CD/UPRT and 0, 10,100, 1 000 mg/L concentrations of 5-FC. After 4 days of incubation, MTT assays were performed to measure the cell viability. Resuits The cell killing effects depended on Ad-CD/UPRT dose and the 5-FC concentration. Mixed cellular assay revealed that CD/UPRT-positive cells had a bystander effects on CD/UPRT-negative cells. Conclusion Ad-CD/UPRT and 5-FC system can effectively suppress the pulmonary carcinoma cell growth. |
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| Keywords: | Pulmonary carcinoma Adenovirus Gene therapy |
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