总姜黄素在大鼠体内的药动学研究

[目的]建立大鼠灌胃总姜黄素溶液后血浆中姜黄素的高效液相色谱(HPLC)测定方法,进行大鼠体内药动学研究.[方法]大鼠灌胃总姜黄素溶液后,不同时间眼底静脉丛取血,制备血浆,血浆样品经液-液萃取以后,以大黄素为内标,进行HPLC检测.色谱条件Hypersil C18柱(250mm×4.6mm,5μm),流动相为乙腈-2.0%醋酸水溶液(46:54),检测波长为UV 420 nm.[结果]姜黄素的线性范围为2～300 mg/L,最低定量限为2 mg/L,日内、日间峰面积(RSD)分别≤5.08%和≤7.93%.[结论]此法灵敏、快速、准确,可用于大鼠血浆中姜黄素浓度的测定和临床前药代动力学研究.

In vivo pharmaco kinetics study of total curcumine in rats

[Objective] To carry out a in vivo pharmacokinetics study on total curcumine and establish a HPLC determination method of blood curcumine.[Methods] The plasma of total curcumine was prepared and the content of total curcumine determined.Using Hypersil C₁₈ column (250 mm×4.6 mm,5 um),the mobile phase was a mixture of acetonitrile-acetic acid (46:54) and the wavelength was 420 nm.[Results] The line range of total curcumine was 2~300 ug/L,the lowest limit of quantity was 2 ug/L and RSD was between 5.08% and 7.93%. [Conclusion] The method is simple,accurate and can be used to control the quality of total curcumine and in pre-clinic pharmacokinetics study.