西红花苷对低密度脂蛋白所致鹌鹑内皮细胞损伤的保护作用

目的探讨西红花苷对低密度脂蛋白(LDL)所致内皮细胞损伤的保护作用。方法在体外培养牛主动脉内皮细胞中,加入不同剂量的西红花苷培养12 h后,再加入50μg.ml-1的LDL继续培养24 h,测定培养液中NO含量,测定细胞中一氧化氮合酶(NOS)的活性。在鹌鹑饮食性动脉粥样硬化模型上,观察西红花苷连续9周预防性给药对血清LDL和血清NO的影响。结果西红花苷可浓度依赖性地提高细胞内NOS的活性(P<0.01),使细胞合成分泌的NO含量升高(P<0.01)。西红花苷能显著降低血清LDL水平,使血清NO含量提高。结论西红花苷对LDL所致内皮细胞损伤有保护作用。

Protective effects of crocin on cultured calf aortic endothelial cells injured by low- density lipoproteins

OBJECTIVE To study protective effects of crocin on cultured calf aortic endothelial cells injured by low-density lipoproteins(LDL).METHODS Bovine aortic endothelial cells was incubated with crocin for 12 hours at different dosages,and then cultured with LDL for 24 h,the activity of LDH,NO in culture media and activity of NOS in endothelial cells were measured.The atherosclerosis formation was induced by hyperlipidamic diet,after the 9 weeks,the leves of serum LDL and NO were measured.RESULTS Compared with control,LDL group could decrease activity of NO in culture media and activity of NOS in endothelial cells.If endothelial cells was preincubated with crocin,the effects of LDL could be inhibited.Compared with the model group,crocin was reduce the level of LDL and elevation NO concentration.CONCLUSION It indicates that crocin has protective effects on cultured calf aortic endothelial cells injured by low-density lipoproteins.