| 摘 要： | AIM:To investigate the rrelationship between methylationof Syk(spleen tyrosine kinase)gene in promoter regionand oncogenesis,metastasis of gastric carcinoma.Therelation between silencing of the Syk gene and methylationof Syk promoter region was also studied.METHODS:By using methylation-specific PCR(MSP)technique,the methylation of Syk promoter region inspecimens from 61 gastric cancer patients(tumor tissuesand adjacent normal tissues)was detected.Meanwhile,RT-PCR was used to analyse syk expression exclusively.RESULTS:The expression of the Syk gene was detectedin all normal gastric tissues.Syk expression in gastriccarcinoma was lower in 14 out of 61 gastric cancer samplesthan in adjacent normal tissues(X~2=72.3,P<0.05).Nomethylation of Syk promoter was found in adjacent normaltissues,hypermethylation of Syk gene in promoter wasdetected 21 cases in 61 gastric carcinoma patients.Therate of methylation of Syk promoter in gastric carcinomawas higher than that in adjacent normal tissues(X~2=25.1,P<0.05).In 31 patients with lymph node metastasis,17 werefound with Syk promoter methylation.A significant differencewas noted between two groups(X~2=11.4,P<0.05).CONCLUSION:Hypermethylation leads to silencing of theSyk gene in human gastric carcinoma.Methylation of Sykpromoter is correlated to oncogenesis and metastasis ofgastric carcinoma.Syk is considered to be a potential tumorsuppressor and anti-metastasis gene in human gastric cancer.
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