肝硬变内HBV DNA及其五种抗原的表达及意义

取２２５例人肝硬变活检组织石蜡切片，检测了ＨＢＶＤＮＡ及其５种抗原。分别用免疫组化ＡＢＣ法检测ＨＢｘＡｇ、ｐｒｅ－Ｓ＿１和ｐｒｅ－Ｓ＿２抗原；用ＰＡＰ法检测ＨＢｓＡｇ和ＨＢｃＡｇ；用原位杂交方法检测ＨＢＶＤＮＡ；用免疫组化、原位杂交双标记方法检测ＨＢＶＤＮＡ和ＨＢｓＡｇ、ＨＢｘＡｇ或ＨＢｃＡｇ。结果显示，阳性检出率ＨＢｓＡｇ为７０．０％（１２８／１８３例），ｐｒｅ－Ｓ＿１抗原为６４．４％（８５／１３２例）、ｐｒｅ－Ｓ＿２抗原为６１．４％（８１／１３２例），ＨＢｘＡｇ为７５．３％（１１３／１５０例），ＨＢｃＡｇ为２２．４％（３９／１７４例），ＨＢＶＤＮＡ为６２．４％（５８／９３例）。双标阳性检出率ＨＢＶＤＮＡ和ＨＢｓＡｇ为３７．３％（１９／５１例），ＨＢＶＤＮＡ和ＨＢｘ－Ａｇ为８６．３％（４４／５１例），ＨＢＶＤＮＡ和ＨＢｃＡｇ为３９．２％（２０／５１例）。ＨＢＶＤＮＡ和ＨＢＶ５种抗原阳性病例中８０％以上均伴有肝细胞不典型增生。这一结果表明，在我国肝硬变的发生发展与ＨＢＶ慢性感染有密切的关系。

Expression and significance of HBV DNA and its 5 antigens in liver cirrhosis

epatitis B virus(HBV)DNA and its 5 antigens were studied in 225 cases of paraffin-embedded sections of human liver cirrhosis obtained by biopsy.HBxAg pre-S_1 and pre-S_2 antigens were detected by immunomstochemical ABC method,HBsAg and HBcAg by PAP method,NBV DNA by in situ hy-bridization, and both HBV DNA and HBsAg,HBxAg or HBcAg by double labelling technique of im-munohistochemistry and in situ hybridization respectively. The results showed that the positive rates were 70.0%(128/183)for HBsAg,64.4%(85/132)for pre-S_1 antigen, 61.4%(81/132) for pre-S_2 antigen, 75.3%(113/150) for HBxAg,22.4%(39/174) for HBcAg and 62.4%(58/93) for HBV DNA respectively. The double labelling positive rates were 37.3%(19/51 ) for both HBV DNA and HBsAg,86.3%(44/51) for both HBV DNA and HBxAg and 39.2%(20/51) for both HBV DNA and HBcAg respectively. More than 8 0%of the cases with positive sections for HBV DNA and its 5 anti-gens were associated with liver cell dysplasia(LCD).The results of this study suggest that the occur- rence and development of liver cirrhosis were closely related to chronic infection of HBV in China.