| 发酵液中重组人血清白蛋白的纯化 |
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| 引用本文： | 陈光明,周长林,魏元刚,赵明龙. 发酵液中重组人血清白蛋白的纯化[J]. 药物生物技术, 2003, 10(1): 25-28 |
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| 作者姓名： | 陈光明 周长林 魏元刚 赵明龙 |
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| 作者单位： | 1. 中国药科大学生物制药学院,南京,210009
2. 上海美通生物科技有限公司,上海,200235 |
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| 摘 要： | 采用摇瓶培养重组毕赤氏酵母（Pichia pastoris）表达并分泌重组人血清白蛋白（recombinant human se-rum alburmin，rHSA）至胞外，发酵液经（NH4）2SO4沉淀、离子交换层析、疏水层析和凝胶过滤分离纯化rHSA，纯化样品经SDS-PAGE检测为单一条带。
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| 关 键 词： | 重组人血清白蛋白 纯化 发酵液 |
| 文章编号： | 1005-8915(2003)01-0025-04 |
Purification of Recombinant Human Serum Albumin from Fermentation Broth |
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| CHEN Guang-ming,ZHOU Chang-lin,WEI Yuan-gang,ZHAO Ming-long. Purification of Recombinant Human Serum Albumin from Fermentation Broth[J]. Pharmaceutical Biotechnology, 2003, 10(1): 25-28 |
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| Authors: | CHEN Guang-ming ZHOU Chang-lin WEI Yuan-gang ZHAO Ming-long |
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| Abstract: | Recombinant human serum albumin (rHSA) was expressed and secreted into the fermentation broth by the cultivation of recombinant Pichia pastoris. It was then isolated and purified by the precipitation with (NHt)2SO4, ion exchange chromatography, hydrophobic chromatography and gel filtration chromatography respectively. SDS-PAGE demonstrated that the purified rHSA was a single band. |
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| Keywords: | Recombinant human serum albumin Purification |
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