职业性慢性铅中毒患者T细胞亚群及Th1/Th2细胞因子的变化

目的初步探索职业性慢性铅中毒患者T淋巴细胞亚群及Th淋巴细胞因子的变化特点。方法对23例职业性慢性铅中毒患者（铅中毒组）及20例健康非职业铅接触成人（对照组）采用流式细胞技术检测外周静脉血淋巴细胞CD3、CD4、CD8的表达，同时通过流式细胞微球芯片捕获技术检测血浆IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ的浓度。结果铅中毒患者外周静脉血CD4相对百分比（32．68％±11．54％）及CD4／CD8比值（0．89±0．39）与对照组比较，明显下降，差异有统计学意义（P〈0．05）；血浆IL-2浓度为（2．00±0．68）pg／ml，与对照组比较明显上升，差异有统计学意义（P〈0．05）；血浆IL-10和IFN-γ浓度分别为（1．83±0．85）pg／ml和（3．42±0．85）pg／ml，与对照组比较明显减低，差异有统计学意义（P〈0．05）。结论T淋巴细胞亚群及细胞因子对评价铅免疫毒性有进一步研究价值。

Changes in T lymphocyte subsets and plasma Th1/Th2 cytokine levels in patients with occupational chronic lead poisoning

OBJECTIVE: To investigate the changes in T lymphocyte subsets and plasma Th1/Th2 cytokine levels in patients with occupational chronic lead poisoning. METHODS: Flow cytometric analysis was used to count the numbers of CD3+, CD4+, CD8+ cells and cytometric bead array CBA capture technique was used to quantify plasma IL-2, IL-4, IL-6, IL-10, TNF-alpha, IFN-gamma concentrations. Twenty-three patients with occupational chronic lead poisoning and 20 controls were examined. RESULTS: Compared with control group, CD3+ CD4+ lymphocytes and CD4/CD8 ratios decreased in patients with occupational chronic lead poisoning. The plasma IL-2 level were significantly raised while the IL-10 and IFN-gamma were reduced in patients with occupational chronic lead poisoning. CONCLUSIONS: T lymphocyte subsets and plasma Th1/Th2 cytokine levels could be a new parameter to assess lead immunotoxity.