重症肌无力患者血清兰尼定碱受体抗体的测定及临床意义

目的探讨血清兰尼定碱受体抗体测定对于重症肌无力诊断的临床意义。方法采用梯度离心法纯化兔骨骼肌肌浆网作为兰尼定碱受体抗体的抗原,ELISA法测定74例重症肌无力患者（其中21例合并胸腺瘤）血清中兰尼定碱受体抗体的水平,并比较其临床意义。结果Western blot验证梯度离心纯化的肌浆网中存在兰尼定碱受体,血清受体抗体测定在重症肌无力合并胸腺瘤患者中的阳性率较高,与重症肌无力病情严重程度密切相关。结论血清兰尼定碱受体抗体水平检测对于重症肌无力合并胸腺瘤诊断及判定预后具有重要临床意义。

Determination and clinical significance of serum anti-ryanodine receptor antibody in patients with myasthenia gravis

Objective To explore the clinical significance of serum anti-ryanodine receptor(RyR)antibody in the diagnosis of myasthenia gravis(MG).Methods The crude sarcoplasmic reticulum was prepared from rabbit skeletal muscle,and then purified by differential centrifugation to produce the antigen.The serum anti-RyR antibody levels in 74 patients with MG(including 21 patients with comorbidic thymomas)were determined with ELISA.Results Western blot demonstrated the presence of RyR in purified crude sarcoplasmic reticulum.The positive rate of anti-RyR antibody was significantly higher in MG patients who had comorbidic thymoma compared with those who had no such comorbidity(P<0.01).Also,the positive rate was closely correlated with the severity of MG.Conclusion Serum anti-RyR antibody test is helpful in the diagnosis of MG associated with thymoma and can be used to judge the outcome of MG.