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| 丙型肝炎患者血清中的交叉中和抗体 | |
| 引用本文： | 严石,童一民,华显,魏萍,尹迪,赵平,戚中田.丙型肝炎患者血清中的交叉中和抗体[J].中华实验和临床感染病杂志（电子版）,2008,2(3):131-137. |
| 作者姓名： | 严石 童一民 华显 魏萍 尹迪 赵平 戚中田 |
| 作者单位： | 1. 150030,哈尔滨市,东北农业大学;第二军医大学微生物学教研室 2. 第二军医大学微生物学教研室 3. 东北农业大学,哈尔滨市,150030 |
| 基金项目： | 上海市基础重点项目(06JC14084);;国家自然科学基金(30671921,30770094);;国家“863”基金(2006AA02Z401) |
| 摘 要： | 目的 分析HCV感染者血清中是否存在交叉反应性中和抗体.方法 以分泌表达HCV包膜E2蛋白真核表达质粒转染的293T 细胞培养上清液中的HCV E2蛋白作为检测抗原,建立检测HCV E2抗体的ELISA方法,检测48份HCV抗体阳性的慢性丙型肝炎患者血清,然后用免疫荧光分析血清与HCV全长包膜蛋白表达质粒转染的293T细胞的结合反应,再用5株HCV假病毒为模型分析阴性血清的病毒中和活性.结果 48份抗-HCV阳性血清中,E2抗体阳性血清达36份,阳性率75%.免疫荧光分析的结果与ELISA检测一致.HCV E2抗体阳性血清对5株HCV假病毒的感染性均有不同程度的中和作用,中和活性与E2抗体水平相一致.结论 丙型肝炎患者血清中存在交叉中和抗体,提示开发能诱导广泛交叉中和抗体的丙型肝炎疫苗或许具有可行性. |
| 关 键 词： | 丙型肝炎 中和抗体 E2蛋白 |
Cross-neutralization antibodies against HCV in sera of patients with hepatitis C |
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| YAN Shi,TONG Yi-min,HUA Xian,WEI Ping,YIN Di,ZHAO Ping,QI Zhong-tian.Cross-neutralization antibodies against HCV in sera of patients with hepatitis C[J].Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version),2008,2(3):131-137. | |
| Authors: | YAN Shi TONG Yi-min HUA Xian WEI Ping YIN Di ZHAO Ping QI Zhong-tian |
| Abstract: | Objective To study whether cross-neutralization antibodies against HCV are present in sera of hepatitis C patients.Methods A eukaryotic expression plasmid encoding carboxyl terminal-truncated HCV envelope protein 2(E2) of H77 strain was constructed and transfected into human 293T cells.Both intracellular E2 and secreted E2 protein in supernatant could be detected by Western blot analysis with anti-E2 McAb.The supernatant was used to assay anti-E2 antibodies in sera of hepatitis C patients by an ELISA kit.Th... |
| Keywords: | Hepatitis C Neutralization antibodies Envelope protein 2 |
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