三叶青对小鼠免疫性肝损伤保护作用的研究

目的:探讨三叶青对卡介苗(BCG)和脂多糖(LPS)诱导的小鼠免疫性肝损伤的保护作用。方法:静脉注射BCG和LPS诱导小鼠免疫性肝损伤。在注射LPS前,小鼠腹腔注射不同剂量的三叶青(10、15、20mL/kg),连续10d后,取血清,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)的活性,测定肝组织匀浆中脂质过氧化产物丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:不同剂量的SYL治疗组小鼠血清ALT、AST、LDH活性及肝组织匀浆MDA含量均低于模型组,且肝组织损伤不同程度地减轻。结论:三叶青对BCG和LPS诱导的小鼠免疫性肝损伤具有一定的保护作用。

The Study on Protective Effect of Sanyeqing on Experimental Liver Injury in mice

Objective： To study the protective effect of Sanyeqing （SYQ）on experimental liver injury induced by Calmette-Guerin bacillus vaccine （BCG） and Lipopolysaccharide （LPS） in mice. Methods： Experimental liver injury was induced by Calmette-Guerin bacillus vaccine and Lipopolysaccharide in male mice by injecting via tail vein （50μg/kg）, and administering LPS （10μg/mouse） 10d later. Therapeutic groups were given respectively with SYQ （10,15,20 mL/kg） before administration of LPS. The level of ALT,AST and LDH in serum were measured by autooxdation method.and the level of SOD was measured by pymgallol autooxdation method. Results： The three experimental groups were found with significant decrease in the elevation of serum ALT, AST and LDH level and the content of MDA in the liver plasma. The liver tissue damages were also ameliorated. Conclusion： SYQ has protective effect against the experimental liver injury induced by BCG and LPS in mice.