丙型肝炎病毒核酸疫苗的构建及小鼠体液免疫效果

目的 探讨核酸商以苗在HCV感染中的预防和治疗价值。方法 将HCV C区基因插入pEF质粒EF－1α下游，构建pEF-HCV C重组质料，然后经im及皮下注射免疫BALB／c小鼠，免疫次数分别为1，2，3，4次，ELISA法检测免疫小鼠血清中抗－HCV的水平。结果 接种后各组均检测到抗－HCV的水平。结果 接种后各组均检测到抗－HCV，但是抗体滴度较低，在不同的免疫次数中，4次免疫组抗体水平最高。结论 构建的HCV DNA重组体可诱发BALB／d小鼠产生体液免疫应答，加强免疫后可使血清中抗－HCV明显增高。

Construction of hepatitis C virus DNA vaccine and its effects in mice

AIM　To study the protective and therapeutic value of DNA vaccine in HCV infection. METHODS　The recombinant plasmid pEF-HCV was constructed by inserting the highly conserved HCV-core gene fragment into the eukaryotic expression vector pEF under the control of an EF-1α promoter, and then was injected into the quadriceps muscles or inoculated subcutaneously into right and left flank of BALB/c mice. Antibody to the HCV core protein in serum was detected by ELISA. RESULTS　Antibody to HCV core protein was detected in serum of all the mice receiving injection of recombinant pEF-HCV-C, but the titers were low. The serum antibody level rose significantly after the booster injection, the antibody levels of mice with four times inoculation were the highest. CONCLUSION　The results suggested that HCV core DNA-based immunization can induce HCV specific antibody, but the humoral immune response was weak at early stage, and the booster immunization can induce the rise of the antibody significantly.