| 重组人MUC1-MBP融合蛋白诱导小鼠Th1活化 |
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| 引用本文： | 张淑芳,台桂香,马吉春,高航. 重组人MUC1-MBP融合蛋白诱导小鼠Th1活化[J]. 免疫学杂志, 2007, 23(4): 373-375 |
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| 作者姓名： | 张淑芳 台桂香 马吉春 高航 |
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| 作者单位： | 长春市医学高等专业学校生化室;吉林大学基础医学院免疫学教研室,长春,130021 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 目的 检测重组MUC1-MBP融合蛋白对小鼠Th1细胞的活化作用.方法 通过生物活性法测定MUC1-MBP免疫鼠脾细胞培养上清液中IL-2、IFN和血清中TNF水平;通过免疫组织化学染色法检测CD4 T细胞在肿瘤组织中的浸润.结果 MUC1-MBP免疫鼠脾细胞分泌IL-2和IFN及血清TNF水平较对照组明显增高;MUC1-MBP诱导小鼠CD4 T细胞在肿瘤组织中浸润.结论 重组人MUC1-MBP融合蛋白可激活小鼠Th1细胞.
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| 关 键 词： | MUC1-MBP融合蛋白 Th1细胞 细胞因子 |
| 文章编号： | 1000-8861（2007）04-0373-03 |
| 修稿时间： | 2006-05-292006-12-05 |
Recombinant MUC1-MBP fusion protein activates mouse Th1 cells |
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| ZHANG Shu-fang,TAI Gui-xiang,MA Ji-chun,GAO Hang. Recombinant MUC1-MBP fusion protein activates mouse Th1 cells[J]. Immunological Journal, 2007, 23(4): 373-375 |
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| Authors: | ZHANG Shu-fang TAI Gui-xiang MA Ji-chun GAO Hang |
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| Affiliation: | Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China |
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| Abstract: | Objective To investigate the activity of Th1 cells in mice immunized with recombinant MUC1-MBP fusion protein.Methods The levels of IL-2 and IFN secreted by splenocytes and the serum TNF were detected in mice immunized by recombinant MUC1-MBP.Immunohistochemistry technique was used to analyze the infiltration of CD4+ T cells into cancerous tissue of MUC1-MBP immunized mice.Results The levels of IL-2 and IFN secreted by splenocytes and the serum TNF were higher in MUC1-MBP-immunized mice than those in the groups of negative control.MUC1-MBP induced the infiltration of CD4+ T cells into cancerous tissue.Conclusion The recombinant MUC1-MBP fusion protein can activate Th1 cells of mouse. |
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| Keywords: | MUC1-MBP fusion protein Th cells Cytokine MUC1 Mucins |
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