菊苣化学成分的LC-MS／MS定性分析与HPLC含量测定

目的建立菊苣化学成分的定性、定量分析方法,完善药材质量控制手段。方法以HPLCDAD-ESI-MS方法,对菊苣化学成分进行定性鉴定,并采用HPLC方法同时测定菊苣中5种化学成分含量。结果从菊苣中共鉴定了10个化学成分,其中6个成分的保留时间和质谱碎片离子信息与对照品一致,分别为秦皮甲素、绿原酸、秦皮乙素、菊苣酸、异绿原酸B、异绿原酸A;根据质谱碎片信息并结合文献数据初步确定了另外4个成分的化学结构。建立了菊苣中绿原酸、秦皮乙素、菊苣酸、异绿原酸B、异绿原酸A的回归方程,线性关系均良好(r0.999 5),加样回收率为95.94%~104.41%,采用此方法成功检测了10个菊苣样品中上述5种成分的含量。结论研究建立的方法可定性、定量分析菊苣中的主要化学成分,可用于菊苣药材的质量分析。

Qualitative and quantitative analysis of Chicory Root by LC/MS and HPLC

Objective To establish the methods for identification and determination of chemical components in Chicory (Herba Cichorii Radix Cichorii,Juju)to optimize the quality control methods of Chinese medicinal materials.Methods High performance liquid chromatography (HPLC)-diode array detctor (DAD)-electrospray ionization mass spectrometry (ESI-MS)was used for separation and charac-terization,and HPLC for simultaneous determination of five active components in Chicory.Results Six of ten components identified were aesculin,chlorogenic acid,esculetin,chicoric acid,chlorogenic acid B and chlorogenic acid A,in line with the features of retention time and MS fragment ions,four of ten were preliminarily determined their chemical constitutions by MS /MS spectra and data from literature.In addition,the regression equations of chlorogenic acid,esculetin,chicoric acid,chlorogenic acid B and chlorogenic acid A were established and showed good linear relationship(r >0.999 5)with the recovery of standard additon of 95.94% ~104.41%.The contents of the five components were determined using HPLC.Conclusion The method established could rapidly separate,identify and measure the bioactive components of Chicory Root,which can be proposed to control the quality of medicinal material.