LIGHT／INF—γ基因配比转染HepG2细胞对其凋亡及Fas／FasL系统表达的影响

目的：观察脂质体介导的LIGHT和IFN—γ配比转染HepG2细胞后对其凋亡及Fas和FasL表达的影响。方法：将HepG2细胞分为LIGHT单独转染组、LIGHT和IFN—γ联合转染组和空白对照组，以脂质体为中介转染HepG2细胞；分别于转染后12、24和48h收集HepG2细胞，流式细胞术检测转染后HepG2细胞的凋亡率及Fas和FasL的表达。结果：LIGHT基因转染HepG2细胞能明显促进其凋亡，随时间延长凋亡率增加；Fas和FasL在HepG2细胞高表达，以Fas升高程度显著。结论：LIGHT和IFN—γ联合转染HepG2，其凋亡效果优于LIGHT单独转染，主要是通过上调Fas的表达来促进HepG2细胞凋亡。

Expression of Fas/FasL and the apoptosis of HepG2 cells transfected with LIGHT and IFN-γ

Objective To investigate the expression of Fas, FasL and the apoptosis of liver cancer cell line HepG2 transfected with LIGHT and IFN-γ gene mediated by Cationic liposome. Methods HepG2 cells were divided into two groups （the solo transfection of LIGHT gene and the combined trans- fection of LIGHT and IFN-γ genes） and the control groups （no transfection）. HepG2 cells were cellected at 12h, 24h and 48h after transfection. The apoptosis of HepG2 cells and the expression of Fas and FasL of the HepG2 cells were investigated with flow cytometry. Results =After transfection, the apoptosis of HepG2 cells increased, and the apoptosis of combined transfection group was higher than the solo transfection of LIGHT（P〈 0.01 ）. The expression of Fas and FasL increased in HepG2 cells（P 〈 0.01）, but the expression of FasL was lower than that of Fas. Conclusion The effect of the combined transfection of LIGHT and IFN-γ genes is better than that of the solo transfection of LIGHT gene. LIGHT and IFN-γ can significantly promote cell apoptosis by regulating the expression of Fas.