薄壁滤过泡对左氧氟沙星滴眼液进入兔眼晶状体的影响

目的研究兔眼薄壁滤过泡形成后局部滴用左氧氟沙星滴跟液晶状体内浓度的变化。设计实验研究。研究对象新西兰白兔。方法将右眼行联合丝裂霉素C的巩膜咬切术后形成苍白、隆起、薄壁滤过泡的新西兰白兔26只，随机分为A、B、C三组。其中A、B组各8只，每日4次双眼滴左氧氟沙星滴眼液，每次0．1ml，分别持续4天和7天；C组10只，每30分钟双眼滴左氧氟沙星滴眼液一次，共点8次。i组全部最后一次滴左氧氟沙星滴眼液半小时后处死兔，取出晶状体，通过高效液相法（HPLC）检测晶状体中左氧氟沙星的浓度，分别比较各组中薄壁滤过泡眼与自身健康对照眼晶状体左氧氟沙星浓度的差别，比较各组之间薄壁滤过泡眼品状体左氧氟沙星浓度的差别。主要指标兔眼晶状体中左氧氟沙星浓度。结果三组中薄壁滤过泡眼（A组：0．33±0．10μg／g；B组：0．27±0．15μg／g；C组：0．62±0．28μg／g）与自身健康对照眼（A组：0．12±0．03μg／g；B组：0．12±0．03μg/g；C组：O．15±0．05μg／g）晶状体中左氧氟沙星浓度差异有统计学意义（A组：P〈0．0001；B组：P〈0．05；C组：P〈0．0001），A组和B组薄壁滤过泡跟晶状体左氧氟沙星浓度差异无统计学意义（P=0．566），A、B组与C组薄壁滤过泡眼晶状体左氧氟沙星浓度差异有统计学意义（P=0．006；0．001），C组（0．62±0．28μg坛）薄壁滤过泡眼晶状体中左氧氟沙星浓度分别高于A组（0．33±0．10μg／g）和B组（0．27±0．15μg／g）。薄壁滤过泡结膜下大片疏松区域，结膜上皮萎缩变薄，结膜基底层细胞不规则，间隙增宽，炎症细胞浸润。结论薄壁滤过泡形成后，眼内晶状体中左氧氟沙星浓度增高，频繁点药品状体内左氧氟沙星浓度增高更为明显。

Penetration of topically applied levofloxacin into rabbit lens with thin-wall filtering bleb after filtering surgery

Objectives To investigate the thin-walled bleb penetration of topical levofloxacin into the rabbit lens. Design Experimental study. Participants New Zealand white rabbit. Methods Twenty-six New Zealand white rabbits with thin-walled blebs after filtering surgery to right eyes were divided randomly into three groups A, B, C. Eight rabbits in each group A and B, and all eyes received 0.1ml of 0.3% levofloxacin 4 times every day, which last tor 4 days in group A and 7 days in group B. Ten rabbits with 20 eyes in group C, and 0.1ml of 0.3% levofloxacin was administrated to the eyes at 30 minute intervals for 4 hours. All the rabbits received the hast drop administered 30 minutes befnre been put to death. The eoneentration of levofloxacin in lens was determined by high-performance liquid chromatography （HPLC）. The Student＇s t-test and Chi-square test were used to compare the data of the three groups. A P〈0.05 was required for results to be considered statistically significant. Main Outcome Measures The concentration of levofloxacin in rabbit lens. Results The concentration of levofloxacin in the lens of thin-walled bleb eyes was 0.33±0.10 μg/g, 0.27±0.15 μg/g and 0.62±0.28 μg/g in groups A, B and C and 0.12±0.03 μg/g, 0.12±0.03 μg/g and 0.15±0.05 μg/g in control eyes of groups A, B and C, and the difference of levofloxaein concentration in lens between brother eyes in each group was statistically significant （group A： P〈 0.0001 ;group B： P〈0.05; group C： P〈0.0001. The difference of levofloxacin concentration in the lens of thin-walled bleb eyes between group A and B was non-statistically significant （P=0.566）. But it was statistically significant between either group A and C （P=0.006）, or group B and C （P=0.001）. Conclusions Levofloxacin penetrate into rabbit lens better in thin-walled bleb group than in control group, and it seemed that higher concentration of levofloxacin in lens occurred by high frequency administration.