雄芍汤对免疫性肝纤维化大鼠MMP-9和TIMP-1的影响

目的:研究雄芍汤对肝纤维化大鼠基质金属蛋白酶-9(MMP-9)和金属蛋白酶组织抑制因子-1(TIMP-1)的影响.方法:大鼠分为正常组、模型组、扶正化瘀胶囊对照组(0.525 g·kg-1)、雄芍汤预防组(7.9 g·kg-1)和高、低剂量组(15.8,7.9g·kg-1).采用猪血清腹腔注射法复制大鼠肝纤维化模型.酶联免疫吸附(ELISA)法检测大鼠血清MMP-9含量,实时荧光定量PCR技术检测肝组织中TIMP-1 mRNA的表达.结果:雄芍汤7.9 g·kg-1剂量预防组MMP-9含量显著高于模型组(P＜0.01).雄芍汤15.8 g·kg-1剂量组TIMP-1 mRNA表达显著低于模型组(P＜0.01).结论:雄芍汤可以促进免疫性肝纤维化大鼠血清MMP-9生成,抑制大鼠肝组织TIMP-1 mRNA表达.调节MMP与TIMP的协调平衡可能是雄芍汤抗肝纤维化的机制之一.

Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis

Objective: Tostudy the effects of Xiongshao decoction (XSD)on matrix metalloproteinase 9(MMP-9) and tissue inhibitor of metalloproteinases 1(TIMP-1)in rats with liver fibrosis. Method: Eighty-four SD rats were randemly divided into normal group, model group, Fuzheng Huayu capsule group(0.525 g·kg^-1), XSD preservation group(7.9 g·kg^-1),high and low doses of XSD group(15.8,7.9 g·kg^-1).The hepatic fibrosis model was induced by injecting porcine serum to the abdominal cavity of rats. The levels of MMP-9 was detected by ELISA. The content of TIMP-1mRNA in hepatic tissue was detected by real-time fluorescence quantitative PCR. Result: The content of MMP-9 was obviously higher in XSD preservation group(7.9 g·kg^-1) than in model group(P<0.01).The content of TIMP-1mRNA was obviously lower in high-dose XSD group(15.8 g·kg^-1) than in model group(P<0.01). Conclusion: XSD can promote the production of MMP-9 and suppress the expression of TIMP-1 mRNA.Regulating the balance between MMPs and TIMPs is one of the anti-hepatic fibrosis mechanisms of XSD.