响应面法优化松花粉总酚的超声提取工艺

目的:优选松花粉中总酚的超声提取工艺.方法:以总酚得率为指标,采用单因素试验考察乙醇体积分数、超声时间、超声温度、料液比等因素对总酚得率的影响,在此基础上,运用响应面法优选总酚超声提取工艺.结果:松花粉总酚的最佳超声提取工艺条件为30倍量39％乙醇于51℃下超声提取24 min,在此条件下,总酚得率3.85 mg·g-1,实测值与模型预测值相符.结论:响应面分析法可较好地用于优化松花粉总酚超声提取工艺,且该优选工艺方便、快捷、高效.

Optimization of Ultrasonic-assisted Extraction Technology for Total Phenols from Pinus massoniana by Response Surface Methodology

Objective:To optimize ultrasonic-assisted extraction technology of total phenols from Pinus massoniana by response surface methodology.Method: With yield of total phenols as index,the concentration of ethanol,ultrasonic time,ultrasonic temperature and solid-liquid ratio were investigated by single factor test,based on this,ultrasonic extraction technology of total phenols was optimized by response surface methodology.Result: Optimal extraction conditions were:ultrasonic extracted 24 min with 30 times the amount of 39% ethanol at 51 ℃,under these conditions,yield of total phenols reached 3.85 mg · g-1,which was agreed with model predictions.Conclusion: Ultrasonic-assisted extraction technology was simple,rapid,and efficient,response surface method could used well to optimize ultrasonic-assisted extraction of total phenols from P.massoniana.