HPLC-ELSD测定盾叶薯蓣中伪原薯蓣皂苷的含量

目的:建立盾叶薯蓣中伪原薯蓣皂苷的含量测定方法.方法:采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),Dikma C18色谱柱(4.6 mn×250 mm,5μm),流动相乙腈-水(30:70),流速1.0 mL· min-1；蒸发光散射检测器参数:漂移管温度60℃,气压4.5 kPa.结果:伪原薯蓣皂苷在10 ～40 μg(r =0.9998)线性关系良好,平均回收率98.7％ (RSD 1.6％,n=6).结论:该方法简便、快速、灵敏、准确,可作为盾叶薯蓣的质量控制方法.

HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis

Objective:To establish a method for the determination of pseudoprodioscin in Dioscorea zingiberensis.Method:HPLC-ELSD was used in the quantitative analysis with Dikma C18 chromatography column(4.6 mm×250 mm,5 μm) and acetonitrile-water(30∶70) as a mobile phase.The flow rate of mobile was 1.0 mL·min-1;the drifting tube temperature of the detecor was set at 60 ℃.The pressure of pure air was 4.5 kPa.Result: The linear range for pseudoprodioscin was 10-40 μg(r=0.999 8).The average recovery was 98.7%(RSD 1.6%,n=6).Conclusion: This method is simple,rapid,sentive,accurate,and can be regarded as the quality control method of D.zingiberensis.