五加生化胶囊的GC-MS指纹图谱研究

目的:建立五加生化胶囊的气相色谱-质谱联用(GC-MS)法测定指纹图谱.方法:采用GC-MS联用技术对11批五加生化胶囊的进行分析,5HP-MS色谱柱,载气He,流速1.0 mL· min-1,进样量1μL,分流比1:20.质谱条件:电子轰击(EI)离子源,辅助线温度280℃,离子源温度230℃,四级杆温度150℃,质量扫描范围m/z 30～600.采用Chemstation和Nist05a谱库.结果:分析了11个批次的五加生化胶囊,建立了五加生化胶囊GC-MS的指纹图谱,确定了7个共有色谱峰,鉴定其中6个色谱峰.采用中药色谱指纹图谱相似度评价系统软件(2004 A版)进行指纹图谱分析,11批五加生化胶囊样品相似度均在0.90以上.结论:五加生化胶囊的GC-MS指纹图谱特征性及专属性强,该指纹图谱可作为五加生化胶囊挥发性成分质量控制的稳定方法.

GC-MS Fingerprint of Wujia Shenghua Capsule

Objective:To establish GC-MS fingerprint of Wujia Shenghua capsule.Method: Chromatographic conditions: HP-5MS quartz capillary column(0.25 μm×0.25 mm×30 m);temperature program carrier gas(He),velocity 1.0 mL · min-1,the injection volume 1 μL;split ratio 1 ∶ 20.MS conditions: electron impact(EI) ion source,transmission line temperature 280 ℃,ion source temperature 230 ℃,quadrupole temperature 150 ℃ mass scan range m/z 30-600.And the results were contrasted with Chemstation and Nist 05a.Result: Seven peaks were found in the GC-MS specific chromatogram of volatile oil from Wujia Shenghua capsule,and six main common peaks were appraised.The operating standard of Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004 A) was used to calculate.Similarity of 11 batches of Wujia Shenghua capsule decoction samples was higher than 0.90.Conclusion: The fingerprinting could be used as a method of quality control and provide evidence for the research of effective components of Wujia Shenghua capsule.