不同剂量附子对心衰大鼠心室重构作用的研究

目的:研究不同剂量附子长期使用后心力衰竭大鼠心室重构、神经内分泌、血流动力学水平的变化.方法:结扎90只SD大鼠冠状动脉前降支,4周后形成心衰模型,随机分为模型组、假手术组、卡托普利阳性对照组、附子高剂量组(9 g·kg-1)、附子低剂量组(4.5 g·kg-1),造模成功后即开始给药,观察不同剂量的附子水煎液ig 4周后对慢性心力衰竭大鼠的血流动力学、神经内分泌水平和心室重构等指标的影响,并与卡托普利相对照.结果:90只SD雄性大鼠经造模及血流动力学检测后共有50只完成实验,附子高剂量组左室质量指数(LVMI)比各组都有明显增加,血流动力学指标与其他各组相比下降(P＜0.05),低剂量组与卡托普利组上述2项指标与模型组无差别,血清血管紧张素Ⅱ(AngⅡ)附子高剂量组高于假手术组、卡托普利、附子低剂量组(P＜0.05).结论:高剂量使用附子后心衰大鼠血流动力学和神经内分泌水平恶化,心室重构加重；低剂量附子与卡托普利作用类似,能够改善神经内分泌紊乱.

Effects and Dose-effect Relationship of Aconitl Lateralis Radix in Praeparata on Ventricular Remodeling in Rats with Heart Failure Following Myocardial Infarction

Objective:To investigate different doses of ’Aconiti Lateralis Radix Praeparatat’ in regulating ventricular remodeling,neuroendocrine,hemodynamic in chronic heart failure(CHF) rats.Method: A rat model of CHF was established by ligating left anterior descending coronary artery way,ninety Sprague-Dawley(SD) rats were randomly assigned into sham-operation group,model group,captopril-control group,decoction of Aconiti Lateralis Radix Praeparata low(4.5 g · kg-1),high(9 g · kg-1) dose group,drug intervention was started on 28th day after operation,corresponding drugs was given to each treatment group by gavage for 4 weeks.Meanwhile,the same volume of physiological saline was given to the model group and the sham-operation group by gavage once a day for 4 weeks.The hemodynamic,neuroendocrine,ventricular remodeling data were detect by normal way.Result: Fifty rats were survived after operation and observation of hemodynamic data,left ventricular remodeling and angiotension II in high dose group were greatly deteriorated than model group,captopril group and low dose group(P<0.05).Data of hemodynamic were significantly decreased in high dose group(P<0.05).Angiotension II in captopril and low dose group were lower than model group(P<0.05).Conclusion: High dose usage of Aconiti Lateralis Radix Praeparata may harm hemodynamic,neuoendocrine and ventricular remodeling of CHF rats.Like captpril,low dose usage of ’prepared aconite root’ can moderate neuroendocrine of CHF rats.