黄连解毒汤对神经细胞保护作用活性成分的筛选

目的:探讨黄连解毒汤及其主要成分小檗碱、黄芩苷、巴马汀、栀子苷对过氧化氢(H2O2)、谷氨酸(Glu)所致神经细胞损伤的影响.方法:制备H2O2,Glu损伤入神经母细胞瘤细胞(SH-SY5Y)、小鼠神经母细胞瘤细胞(Neuro-2a)模型,设正常对照、模型、黄连解毒汤、小檗碱、黄芩苷、巴马汀及栀子苷组,筛选浓度为7.812 5,15.625,31.25,62.5,125,250,500 mg·L-1,应用MTT法检测药物对损伤后细胞增殖的影响.结果:黄连解毒汤能够促进SH-SY5Y细胞增殖,适当浓度的栀子苷能够促进SH-SY5Y,Neuro-2a细胞增殖,并对H2O2,Glu损伤的SH-SY5Y细胞有保护作用,对H2O2损伤的Neuro-2a也具有保护作用.结论:栀子苷可能为黄连解毒汤对神经细胞保护作用的有效成分.

Screening of Active Constituents from Huanglian Jiedu Decoction for Protective Effect on Neurocytes

Objective: To study the effect of Huangliang Jiedu decoction and its ingredients berberine, baicalin, palmatine, geniposide on the proliferation of neurocytes injured by H₂O₂ and glutamic acid (Glu). Method: H₂O₂ and Glu were respectively used to induce the injured models of SH-SY5Y and Neuro-2a cell lines. The screening concentration of Huangliang Jiedu decoction, berberine, baicalin, palmatine, geniposide were 7.812 5, 15.625, 31.25, 62.5, 125, 250, 500 mg·L^-1 respectively. MTT analysis was employed to evaluated the proliferation effect of cells after the injury. Result: Huangliang Jiedu decoction enhanced the proliferation of SH-SY5Y cell line. Geniposide in a reasonable concentration promoted the proliferation of SH-SY5Y and Neuro-2a cell lines. It also had the protective effects on SH-SY5Y cell line which were damaged by H₂O₂ and Glu. In addition, geniposide increased the proliferation of Neuro-2a cell line injured by H₂O₂. Conclusion: Geniposide might be the active ingredient of Huangliang Jiedu decoction to protect the injuried neurocyte.