| 樟树花粉泛变应原基因的克隆与序列分析 |
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| 引用本文： | 宋娟娟,张红云,刘志刚,康敏雄.樟树花粉泛变应原基因的克隆与序列分析[J].热带医学杂志,2006,6(4):359-361,370. |
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| 作者姓名： | 宋娟娟 张红云 刘志刚 康敏雄 |
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| 作者单位： | 深圳大学生命科学学院,深圳,518060 |
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| 基金项目： | 国家自然科学基金(No.30070702)，广东省科技重点计划项目(No.2003A3080502)，深圳大学校基金(No.2005028) |
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| 摘 要： | 目的从樟树花粉中克隆泛变应原基因。方法通过对多个植物泛变应原基因进行序列同源性比对,根据保守区域序列设计简并性引物,利用RT-PCR结合RACE技术对樟树花粉中的泛变应原基因进行克隆。结果从樟树花粉中克隆到一个新的基因。序列分析显示:所克隆到的基因与其它植物的泛变应原肌动蛋白结合蛋白基因有很高的同源性,因此认为该基因为泛致敏原基因,命名为Cinc1。其在GenBank数据库中的登录号为DQ335252。结论采用简并引物从樟树花粉中克隆到一泛变应原基因,为进一步研究该变应原奠定了理论基础。
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| 关 键 词： | 泛变应原 樟树 基因克隆 |
| 文章编号： | 1672-3619（2006）04-0359-03 |
| 收稿时间： | 2006-01-17 |
| 修稿时间： | 2006-03-02 |
Cloning and Sequence Analysis of Panallergen Genes from Pollen of Cinnamomum camphora ( L. ) Presl |
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| SONG Juan-juan,ZHANG Hong-yun,LIU Zhi-gang,KANG Min-xiong.Cloning and Sequence Analysis of Panallergen Genes from Pollen of Cinnamomum camphora ( L. ) Presl[J].Journal Of Tropical Medicine,2006,6(4):359-361,370. |
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| Authors: | SONG Juan-juan ZHANG Hong-yun LIU Zhi-gang KANG Min-xiong |
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| Abstract: | Objective To clone new panallergen genes from pollen of Cinnamomum camphora. Methods Sequences of panallergen gene from various species were compared and the conservative domains among the sequences were used for degenerate primer designing. RT-PCR and RACE methods were applied to clone the full length panallergen gene from pollen of Cinnamomum camphora. Results A new cDNA clone was obtained. Sequence analysis showed that this clone shared a high sequence homology with panallergen profilin from other species. The gene, designated as Cin c 1, was regarded as a new panallergen profilin gene. The GenBank accession number for this clone was DQ335252. Conclusion A new panallergen gene Cin c 1 was cloned from pollen of Cinnamomum camphora using degenerate primer. Further study of Cin c 1 may enhance our understanding of this panallergen. |
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| Keywords: | panallergen Cinnamomum camphora gene cloning |
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