6-羟多巴胺化学损毁小鼠外周交感神经对艾灸免疫调节作用的影响

应用 6 OHDA化学切除外周交感神经轴突纤维 ,观察艾灸对正常和CY(环磷酰胺 )免疫抑制小鼠免疫功能的影响。结果 :应用 6 OHDA及CY后 ,小鼠胸、脾指数及IL 1、IL 2含量均低于正常组水平 ;艾灸治疗能提高小鼠免疫功能 ,改善其免疫抑制 ,上述指标均高于各自对照组 ,但仍低于交感神经完整的艾灸组。结果表明 ,6 OHDA损毁外周交感神经末梢后 ,艾灸增强与调节免疫的作用被削弱或部分阻断 ,提示交感神经参与艾灸对免疫的调节。

Effect of Sympathectic Nerve Lesion on Moxibustion Induced Immunoregulation in the Mouse

Objective: To observe the effect of sympathectomy on moxibustion induced immunoregulation in mice. Methods: Experiments including two parts were conducted in 80 mice. Sympathecotomy was performed by intraperitoneal (i.p.) injection of 6 hydroxydopamine (OHDA, 100 mg/kg). The first part of the study (40 mice) was randomly and evenly divided into control group, moxibustion (M) group, 6 OHDA group and 6 OHDA+M group. Immune hypofunction was produced by i.p. injection of cyclophosphamde (CY, 50 mg/kg). The second part (40 mice) was evenly divided into CY, CY+M, CY+6 OHDA and CY+6 OHDA+M groups. "Shenshu"(BL 23) was moxibustioned for 5 min, once daily and continuously for 6 days. Serum IL 1 and IL 2 were detected with radioimmunoassay. Results: ① In the 4 groups of the first part of this study, after injection of 6 OHDA, the thymic index, spleen index, IL 1 and IL 2 levels, particularly the former 3 indexes lowered significantly (P< 0.05). After moxibustion, the thymic and spleen index and IL 2 in 6 OHDA group increased significantly (P<0.05) in comparison with 6 OHDA group, i.e. returned to normal (IL 1 and IL 2) or were close to those of control group (thymic and spleen index). ② In the second part, after injection of CY, the 4 indexes lowered clearly and after additional injection of 6 OHDA, these indexes decreased further. Following moxibustion, the 4 indexes of CY+M group increased significantly in comparison with CY group (P<0.05) and those of CY+6 OHDA+M group increased also considerably in comparison with those of CY+6 OHDA group (P<0.05, 0.01). Conclusion: Moxibustion can raise chemical symqathecotomy or/and CY induced immune hypofunction; and sympathetic nerve participates in moxibustion induced up regulation of the immunofunction.