新生大鼠不同脑区内神经前体细胞生物学特性比较

为了观察海马、中脑和顶叶皮质内神经前体细胞的体外生长特性及其分化后所产生的神经元的形态特征,本研究使用碱性成纤维细胞生长因子(bFGF)体外扩增细胞技术和克隆形成实验分析了神经前体细胞的自我更新特性;采用神经前体细胞和骨髓基质细胞共培养方式,通过免疫细胞化学染色方法,研究了神经前体细胞的分化特点。结果发现:(1)新生大鼠海马、中脑和顶叶皮质内的神经前体细胞,在体外的分裂增殖能力无明显区别;(2)新生大鼠海马、中脑和顶叶皮质内的神经前体细胞在相同的条件下,其后代细胞中,神经元和胶质细胞所占的比例基本相同,但不同脑区内神经前体细胞所产生的神经元的形态却表现出明显不同。这些结果提示新生大鼠不同脑区内神经前体细胞的分化潜能已有了一定限制。

COMPARISON OF BASIC CHARACTERISTICS OF NEURAL PRECURSOR CELLS ISOLATED FROM DIFFERENT ENCEPHALIC REGIONS

The present study aims to observe the characteristics of the neural precursor cells isolated from hippocampus, mesencephalon and cortex of parietal lobe and the morphology of the differentiated neurons. With basic fibrillary growth factor (bFGF)-induced cells and clone generation technique, we analyzed the self-renewal properties of the neuronal precursor cells; Via co-culture of neuronal precursor cells and bone marrow stromal cells, investigated immunocytochemically the differentiation characteristics of the neural precursor cells. The results showed that: (1) No differences were observed for in vitro cloning and subcloning efficiency of neural precursor cells isolated from different encephalic regions of neonatal rats; (2) The percentages of neurons or glia cells among the progeny of the neural precursor cells isolated from hippocampus, mesencephalon and cortex of parietal lobe were similar under identical conditions, the morphology of the differentiated cells from different brain areas, however, were evidently different. These results imply that the multi-potential properties of precursor cells located in different encephalic regions are limited in newborn rats.