一种观察血管内皮损伤与自由基关系的新方法

本文用离体兔胸主动脉淋浴灌流系统探讨了电解克氏液对血管内皮的损伤与自由基的关系。结果显示5 mA直流电作用于克氏液1 min使细胞色素C在550 nm吸光度增加,ACh诱导的内皮细胞EDRF释放明显减少,舒张比值降低(D<0.001),血管壁组织MDA含量升高(p<0.01),SOD活性及6-keto-PGF_(1a)含量下降(p<0.01和p<0.05)。说明用离体血管淋浴式灌注电解性自由基损伤法观察血管内皮损伤与自由基的关系为一简单实用的方法。

A new method to study correlation between endothelium damage and free radicals

A correlation between endothelium dysfunction induced by electrolyzed physiological solution and free radicals was investigated with a superfusion cascade system in the rabbit thoracic aorta. Results showed that release of endothelium-de rived relaxing factor (EDRF) induced by ACh, 6-keto-PGF1α content and SOD activity of the donor vessels were reduced significantly (relaxation ratio: 0.10± SD 0.09 vs 0.99± 0.28, p<0.001; 6-keto PGF1α: 150± SD 42 vs 339± 143 pg/mg wet wt, p<0.05; SOD: 0.23± SD 0.08 vs 0.71± 0.19 U/mgprotein, p<0.01) after Krebs solution was electrolyzed (direct current, 5 mA, 1 min), at the same time, MDA content increased (5.8± 1.2 vs 2.6± 0.4 nmol / mg protein, p<0.01). Captopril (100μmol/L) perfused 5 min before electrolysis could protect endothelium from damage induced by electrolyte. It was concluded that the method of combining superfusion cascade system with electrolysis-induced free radicals is simple and practical for the study of the correlation between endothelium damage and free radicals.