当归药材中水溶性成分指纹图谱的分析

目的:本文收集22个不同产地、不同批次的当归,经形态学鉴定均为正品。建立了当归药材中水溶性成分的HPLC指纹图谱。实现了对当归中29个化学成分色谱峰的分离,获取了反映该药材整体的化学数据。方法:将所获化学数据进行化学模式识别研究。用系统聚类分析将当归样品分为两类,以夹角余弦或相关系数为测度,进行相似度计算。结果:以相似度0.80为限度,即相似度在0.80～1.00为第1类,相似度在0.80以下为第2类,与聚类分析结果一致,两种不同测度的相似度趋势一致。结论:在分析未知样品时,只需计算未知样品与标准样本间的相似度,即可对未知样品进行鉴定、分类和评价。

Study on HPLC fringerprint of angelica sinensis

Objective:22 samples of Angelica Sinensis were collected from different areas.It was identified that all of them were Angelica Sinensis.The HPLC chromatographic fingerprints of Angelica Sinensis were set up.The HPLC chromatographic fingerprints of Angelica Sinensis showing 29 peaks,was established from 22 Angelica Sinensis samples and studied by chemical pattern recognition.Methods:Cluster analysis and similarity analysis were adopted to analyze data of HPLC fingerprints of Angelica Sinensis.The Angelica Sinensis samples were identified with hierarchical clustering analysis and divided into two grades,the first grade and the second grade.According to the results of classification,the similarity analysis method for quality assessment of Angelica Sinensis samples was developed.Results:The similarity was determined by cosine or correlation.The similarity of the first grade was 0.80~1.00,the second grade less than 0.80.The result of similarity analysis was the same as that of cluster analysis.The trend was consistent with two different similarities testing the HPLC fingerprints of Angelica Sinensis.Two different measures were confirmed with each other.The HPLC chromatographic fingerprints similarity analysis method can be used to control the quality of Angelica Sinensis effectively.When unknown samples are analyzed by this method,identification,classification and assessment can be carried out by compared the similarity of standard samples with the unknown ones.Conclusions:.