Naringin-induced p21WAF1-mediated G1-phase cell cycle arrest via activation of the Ras/Raf/ERK signaling pathway in vascular smooth muscle cells |
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Authors: | Eo-Jin Lee Gi-Seong Moon Won-Seok Choi Wun-Jae Kim Sung-Kwon Moon |
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Affiliation: | aDepartment of Food and Biotechnology, Chungju National University, 123 Geomdan-ri Iryu-myeon, Chungju, Chungbuk 380-702, Republic of Korea;bDepartment of Urology, College of Medicine, Personalized Tumor Engineering Research Center, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea |
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Abstract: | The flavonoid naringin has been shown to play a role in preventing the development of cardiovascular disease. However, the exact molecular mechanisms underlying the roles of integrated cell cycle regulation and MAPK signaling pathways in the regulation of naringin-induced inhibition of cell proliferation in vascular smooth muscle cells (VSMCs) remain to be identified. Naringin treatment resulted in significant growth inhibition and G1-phase cell cycle arrest mediated by induction of p53-independent p21WAF1 expression; expression of cyclins and CDKs in VSMCs was also down-regulated. In addition, among the pathways examined, blockade of ERK function inhibited naringin-dependent p21WAF1 expression, reversed naringin-mediated inhibition of cell proliferation and decreased cell cycle proteins. Moreover, naringin treatment increased both Ras and Raf activations. Transfection of cells with dominant negative Ras (RasN17) and Raf (RafS621A) mutant genes suppressed naringin-induced ERK activity and p21WAF1 expression. Finally, naringin-induced reduction in cell proliferation and cell cycle protein was abolished in the presence of RasN17 and RafS621A mutant genes. The Ras/Raf/ERK pathway participates in p21WAF1 induction, leading to a decrease in cyclin D1/CDK4 and cyclin E/CDK2 complexes and in naringin-dependent inhibition of cell growth. These novel and unexpected findings provide a theoretical basis for preventive use of flavonoids to the atherosclerosis disease. |
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Keywords: | Naringin Vascular smooth muscle cell p21WAF1 G1 cell cycle arrest ERK/Ras/Raf |
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