| 北京市门头沟区2018-2020年急性胃肠炎患者诺如病毒基因型分析 |
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| 引用本文： | 刘海涛,曹殿起,张博文,吕秋艳,苏健. 北京市门头沟区2018-2020年急性胃肠炎患者诺如病毒基因型分析[J]. 临床荟萃, 2021, 36(8): 691-695. DOI: 10.3969/j.issn.1004-583X.2021.08.003 |
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| 作者姓名： | 刘海涛 曹殿起 张博文 吕秋艳 苏健 |
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| 作者单位： | 门头沟疾病预防控制中心,北京 102300 |
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| 基金项目： | 十三五”国家科技重大专项——基于全基因组的病毒网络化监测和溯源技术体系研究(2017ZXl0104001-003-001);中美新发再发传染病国际合作项目(1U01GH002224) |
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| 摘 要： | 目的 了解北京市门头沟区急性胃肠炎患者诺如病毒基因型.方法 采用实时荧光定量PCR方法对急性胃肠炎患者粪便标本进行诺如病毒核酸检测,普通RT-PCR法对诺如病毒GⅡ基因群标本的多聚酶和衣壳蛋白区域扩增,对扩增产物序列测定.结果 粪便标本诺如病毒阳性检出率为10.75％,其中GⅡ基因群占95.89％,GI基因群占4.11...
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| 关 键 词： | 诺如病毒 胃肠炎 基因型 |
| 收稿时间： | 2021-07-21 |
Analysis of norovirus genotypes of patients with acute gastroenteritis in Mentougou District of Beijing from 2018 to 2020 |
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| Liu Haitao,Cao Dianqi,Zhang Bowen,Lyu Qiuyan,Su Jian. Analysis of norovirus genotypes of patients with acute gastroenteritis in Mentougou District of Beijing from 2018 to 2020[J]. Clinical Focus, 2021, 36(8): 691-695. DOI: 10.3969/j.issn.1004-583X.2021.08.003 |
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| Authors: | Liu Haitao Cao Dianqi Zhang Bowen Lyu Qiuyan Su Jian |
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| Affiliation: | Mentougou District Centers for Disease Control and Prevention, Beijing 102300, China |
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| Abstract: | Objective To investigate the norovirus genotypes of patients with acute gastroenteritis in Mentougou District of Beijing. Methods Ribonucleic Acid(RNA) of norovirus was detected in fecal samples of acute gastroenteritis patients by real time fluorescent quantitative PCR, and region of polyzyme and capsid protein of type samples of norovirus GⅡ was amplified by general RT-PCR, in order to determine sequence of amplified. Results Positive rate of norovirus was 10.75%, 95.89% for GⅡ gene group and 4.11% for G Igene group were detected. GⅡ gene of norovirus include GⅡ.6 [P7], GⅡ.4 [P16], GⅡ.3 [P12], GⅡ.2 [P16] and GⅡ.17 [P17], variation of each genotype virus was not obvious, and nucleotide homology was 86%-100%. Conclusion There are many genotypes and different recombinant subtypes of norovirus in Mentougou District of Beijing, continuous monitoring of dynamic epidemic characteristics of norovirus recombinant genotypes is helpful to improve ability of early warning for disease prevention and control. |
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| Keywords: | norovirus gastroenteritis genotype |
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