嗅鞘细胞移植对实验性变态反应性脑脊髓炎髓鞘修复及运动功能的影响

目的：观察嗅鞘细胞（OECs）对实验性变态反应性脑脊髓炎（EAE）的髓鞘修复的意义。方法：培养、纯化OECs后，移植组将OECs悬液注入EAE大鼠的侧脑室；假手术组按以上方法注入等量生理盐水；观察二组大鼠运动功能评分、组织病理学变化以及神经元细胞凋亡表达情况。结果：OECs移植组运动功能评分显著优于假手术组。差异有显著意义（P〈0．05）；组织病理切片可见嗅鞘细胞移植组髓鞘修复明显优于假手术组：OECs移植组在各时间点上神经细胞凋亡的表达均明显低于假手术组，差异有显著意义（P〈0．05）。结论：OECs移植可以促进自身免疫性脑脊髓炎的髓鞘修复，改善运动功能，减少神经元细胞凋亡的程度，对神经元有保护作用。

Effect of OECs transplantation on myeline repairment and motor function of experimental allergic encephalomyelitis

Objective:To develop a method for the culture of olfactory ensheathing cells(OECs) and to evaluate the therapeutic effect of OECs transplantation on experimental allergic encephalomyelitis(EAE).Method:OECs were cultured in the olfactory bulb of newborn Wistar rat.OECs suspension was infused into the lateral cerebral ventriculus of EAE rats,and the saline with the same quantity was infused in pseudo-operation group.Afterwards,motor-function grade,histo-pathological change and expression of apoptosis of neuron were observed.Result:The motor-function grade of OEC transplantation group was significantly higher than that of pseudo-operation group(P<0.05).Myeline repairment of OEC transplantation group was markedly better than that of controls in histo-pathological slices.The expression of apoptosis of OEC group was statistically lower than that in the controls at every observation time point(P<0.05).Conclusion:OECs transplantation may promote myeline repairment of EAE,improvement of motor function and decrease of neuron apoptosis.