雌激素诱导大鼠催乳素瘤发生机制的研究

 目的研究雌激素诱发大鼠催乳素(prolactin PRL)瘤的机制.方法制备雌激素诱发的大鼠PRL瘤模型.放免法测定大鼠血清PRL水平.用免疫组化方法显示垂体组织PRL蛋白的表达和分布,用BT-PCR方法检测癌基因c-fos mRNA在各组垂体组织中的表达,以β-actin作为内参照,借助于计算机凝胶成像系统分析表达量.结果用药8周后,根据大鼠垂体重量,以及组织学和免疫组化的改变,证实已诱发出PRL瘤模型.在PRL瘤组中,c-fos的表达量明显高于对照组(P＜0.01).结论雌激素刺激垂体PRL细胞表达c-fos癌基因,在雌激素诱导PRL瘤中起一定的作用.

Mechanism of estrogen in uinduction of rat prolactinoma

Objective To study the mechanism of estrogen in inducing rat prolactinoma.Methods Adult Wistar rats were divided into two groups at random.Rat in control group(n=5)were subscutaneously implanted with a blank implant.Rats in prolactinoma group(n=5) were implanted with estrogen-containing implants.After 8 weeks,all the animals were sacrificed.Each pituitary gland was weighed and collected for histopathological and immunocytochemical study.Serum prolactin(PRL) levels were measured by RIA.c-fos mRNA levels in pituitary tissue were measured by RT-PCR.Results Pituitary weights,PRL levels and PRL(+)cell counts in prolactinoma group were higher than those in control group(P<0.1).Prolactinoma models were developed successfully according to serum PRL level,pituitary weight and immunocytochemical profile.The expression levels of c-fos mRNA in prolactinoma group were obviously higher than those in control group(P<0.01).Conclusion Estrogen stimulates c-fos gene expression,which plays an important role in induction of rat prolactinoma.