巨噬细胞移动抑制因子抗体抑制大肠癌生长与肝转移的研究

目的 观察巨噬细胞移动抑制因子(MIF)抗体对大肠癌生长与肝转移的抑制作用.方法 将CT26细胞接种到BALB/C小鼠项背部获得实体瘤,再对20只BALB/C小鼠行盲肠造疝原位接种瘤块术,术后随机将小鼠分为A、B两组,A组隔天腹腔注射MIF-抗体(400μg/只),B组隔天腹腔注射生理盐水,4周后处死小鼠,观测盲肠原位肿瘤和肝脏情况,并对肝组织进行连续病理切片以观察肝转移情况.ELISA方法检测小鼠血清MIF和MMP-9浓度.结果 MIF-抗体干预的小鼠盲肠原位肿瘤的重量较对照组低(1.60±0.18)g比(2.11±0.25)g,P<0.01],两组间肝脏重量差异无统计学意义(0.93±0.07)g比(0.96±0.07)g,P>0.05],但A组大肠癌肝转移率低于B组(1/10比7/10,P<0.05),且A组血清中MIF和MMP-9的浓度也低于B组(21.15±1.59)ng /L比(35.65±1.34)ng/L,P<0.01;(0.19±0.01)μg/L比(0.28±0.04)μg/L,P<0.01].结论 MIF-抗体腹腔注射不仅抑制大肠癌原位肿瘤的生长,而且降低了大肠癌的肝转移率.

Anti-macrophage migration inhibitory factor antibody inhibits tumor growth and hepatic metastasis in the mouse CT26 colon tumor model

Objective To study the influence of anti-macrophage migration inhibitory factor antibody (anti-MIF) on tumor growth and hepatic metastasis of colorectal cancer.Methods After subcutaneous injection of CT26 cells on the nucha of BALB/C mice,the tumor was formed 10 days later.Orthotopic transplantation with fresh tumor tissues was performed on herniated cecum in 20 BALB/C mice,and the mice were randomly divided into group A and group B.The mice in group A and group B received intraperitoneal injection of anti-MIF and normal saline every other day,respectively.Blood samples were collected by heart puncture and the mice were sacrificed 4 weeks later.The tumor in situ and the liver were observed.The whole liver was made into serial slices for observation of the liver metastasis focus.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of MIF and MMP-9 in serum of mice.Results The weight of the tumor in situ of group A was much lighter than that of group B (1.60±0.18) g vs.(2.11±0.25) g,P<0.01].There was no significant difference in liver weight between group A and group B (0.93±0.07) g vs.(0.96±0.07) g,P>0.05].The incidence of hepatic metastasis in group A was significantly lower than in group B 1/10 vs.7/10,P<0.05].Moreover,the expression of MIF and MMP-9 in serum of group A was markedly lower than in group B,too (21.15±1.59) ng/L vs.(35.65±1.34) ng/L,P<0.01;(0.19±0.01) μg/L vs.(0.28±0.04) μg/L,P<0.01].Conclusion Anti-MIF can not only obviously inhibit the growth of colorectal cancer but also decrease the incidence of hepatic metastasis.