| 不同产地半夏及其混淆品的SDS PAGE电泳鉴别 |
| |
| 引用本文： | 黄必胜.不同产地半夏及其混淆品的SDS PAGE电泳鉴别[J].湖北中医学院学报,2006,8(4):13-14. |
| |
| 作者姓名： | 黄必胜 |
| |
| 作者单位： | 湖北中医学院 湖北武汉430065 |
| |
| 摘 要： | 目的建立不同产地半夏与其混淆品的蛋白质电泳鉴别方法。方法采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对不同产地半夏与其混淆品(英山滴水珠、本院药园掌叶半夏、蛇山半夏、钟祥半夏、钟祥掌叶半夏及戟叶犁头尖)进行鉴别分析。结果不同产地的半夏具有相似的电泳图谱,但半夏与其混淆品图谱之间具有显著性差异。结论SDS-PAGE电泳鉴别可以对不同产地的半夏进行初步鉴别,并可鉴别半夏及其混淆品的特征,方法简便、可靠。
|
| 关 键 词： | 半夏 中药鉴别 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 |
| 文章编号： | 1008-987X(2006)04-0013-02 |
| 收稿时间： | 08 7 2006 12:00AM |
| 修稿时间： | 2006年8月7日 |
Distinctiveness of Pinellia Producing in Different Areas and Its False articles by the Method of SDS PAGE Electrophoresis |
| |
| HUANG Bi-sheng.Distinctiveness of Pinellia Producing in Different Areas and Its False articles by the Method of SDS PAGE Electrophoresis[J].Journal of Hubei College of Traditional Chinese Medicine,2006,8(4):13-14. |
| |
| Authors: | HUANG Bi-sheng |
| |
| Institution: | Hubei College of Traditional Chinese Medicine, Wuhan 430065 |
| |
| Abstract: | Objective:To distinguish the albumen component part in the Pinellia producing in different areas and its false-articles by the method of electrophoresis.Method:The sodium dodecyl-sulfate polyacrylamide gel electroporesis technology(SDS-PAGE)was utilized and the electrophoretic mobility were compared among the Pinellia producing in different areas and its false-articles.Results:The electrophoresis graphs of Pinallia producing in different areas like in the same producing areas,while the electrophoresis graphs had remarkable differences among the Pinellia and its false-articles.Conclusion:SDS-PAGE electrophoresis differentiation can offer a reliable,simple and convenient method for protein detection in the Pinellia producing in different areas and its false-articles. |
| |
| Keywords: | Pinellia Identification SDS-PAGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
