Morphine-induced enhancement in the granulocyte response to thioglycollate administration in the rat |
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Authors: | Fecho Karamarie Lysle Donald T |
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Affiliation: | (1) Department of Psychology and Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599;(2) Dental Research Center, The University of North Carolina at Chapel Hill, CB #7455, Chapel Hill, North Carolina, 27599-7455 |
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Abstract: | The present study determined the pharmacological effects of acute morphine treatment on the granulocyte phase of the peritoneal inflammatory response to thioglycollate (TG) in rats. Dual-color flow cytometry using anti-CD11b/c-PE mAb in combination with HIS48-FITC mAb allowed for the determination of morphine's effects on 4 inflammatory cell subsets: CD11b/c+HIS48med granulocytes; CD11b/chiHIS48neg/lo activated macrophages; CD11b/c–HIS48– lymphocytes; and CD11b/c+HIS48hi cells (a monocyte/macrophage and granulocyte subset). Morphine produced a dose-dependent increase in a select subset of inflammatory peritoneal cells, the CD11b/c+HIS48med granulocytes. The effect of morphine was time-dependent, with significant effects first apparent at 4 hr after TG, but the administration of morphine 1 hr before or simultaneously with TG produced a similar increase in CD11b/c+HIS48med granulocytes. Naltrexone completely antagonized the morphine-induced increase in CD11b/c+HIS48med granulocytes. Collectively, these studies show that a single administration of morphine produces a time-dependent, dose-dependent, opioid receptor-mediated enhancement in the peritoneal granulocyte response to TG. |
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Keywords: | opiates peritoneal inflammation granulocytes flow cytometry |
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