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SDS-琼脂糖凝胶电泳分析蛋白尿来源的临床意义
引用本文:周铁成,秦庆,杨小云,徐焰,程晓东,肖鹏涛,张路,郝晓柯.SDS-琼脂糖凝胶电泳分析蛋白尿来源的临床意义[J].现代检验医学杂志,2006,21(4):25-26.
作者姓名:周铁成  秦庆  杨小云  徐焰  程晓东  肖鹏涛  张路  郝晓柯
作者单位:第四军医大学西京医院检验科,陕西,西安,710032
摘    要:目的分析尿蛋白成分来鉴别蛋白尿来源。方法应用十二烷基磺酸钠-琼脂糖凝胶进行非浓缩尿蛋白电泳分析49例蛋白尿患者的尿蛋白成分。结果在肾后性蛋白尿中往往可以检测到巨球蛋白等一类大分子蛋白,而在肾性则往往缺乏。并且以巨球蛋白作为鉴别蛋白尿的标准,其敏感性和特异性均达到95.8%。结论该方法客观性强,诊断符合率高,重复性好,简便快速,值得临床应用。

关 键 词:电泳  琼脂糖凝胶  蛋白尿  巨球蛋白
文章编号:1671-7414(2006)04-025-02
收稿时间:2006-04-07
修稿时间:2006年4月7日

The Clinical Meaning of Electrophoresis Analyzing the Source of the Proteinuria with SDS-agar Gel
ZHOU Tie-cheng,QIN Qing,YANG Xiao-yun,XU Yan,CHENG Xiao-dong,XIAO Peng-tao,ZHANG Lu,HAO Xiao-ke.The Clinical Meaning of Electrophoresis Analyzing the Source of the Proteinuria with SDS-agar Gel[J].Journal of Modern Laboratory Medicine,2006,21(4):25-26.
Authors:ZHOU Tie-cheng  QIN Qing  YANG Xiao-yun  XU Yan  CHENG Xiao-dong  XIAO Peng-tao  ZHANG Lu  HAO Xiao-ke
Abstract:Objective To distinguish the sources of the proteinuria by analyzing the compositions of the urinary protein.Methods Non-concentrated urinary proteins were analyzed with sodium dodecyl sulfate-agarose gel electrophoresis(SDS-AGE)in 49 patients with proteinuria.Results Some kinds of large molecular proteins like macroglobulin could be found in postrenal proteinuria.However,they were generally absent in renal proteinuria.With the method of using macroglobulin as a standard to distinguish proteinuria,the rates of sensitivity and specificity were both 95.8%.Conclusion This method is fast and easy,and has better objective,high accurate of diagnosis,well repeats as well.It deserves to be used.
Keywords:electrophoresis  agar gel  proteinuria  macroglobulin  
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