SDS-琼脂糖凝胶电泳分析蛋白尿来源的临床意义

目的分析尿蛋白成分来鉴别蛋白尿来源。方法应用十二烷基磺酸钠-琼脂糖凝胶进行非浓缩尿蛋白电泳分析49例蛋白尿患者的尿蛋白成分。结果在肾后性蛋白尿中往往可以检测到巨球蛋白等一类大分子蛋白,而在肾性则往往缺乏。并且以巨球蛋白作为鉴别蛋白尿的标准,其敏感性和特异性均达到95.8%。结论该方法客观性强,诊断符合率高,重复性好,简便快速,值得临床应用。

The Clinical Meaning of Electrophoresis Analyzing the Source of the Proteinuria with SDS-agar Gel

Objective To distinguish the sources of the proteinuria by analyzing the compositions of the urinary protein.Methods Non-concentrated urinary proteins were analyzed with sodium dodecyl sulfate-agarose gel electrophoresis(SDS-AGE)in 49 patients with proteinuria.Results Some kinds of large molecular proteins like macroglobulin could be found in postrenal proteinuria.However,they were generally absent in renal proteinuria.With the method of using macroglobulin as a standard to distinguish proteinuria,the rates of sensitivity and specificity were both 95.8%.Conclusion This method is fast and easy,and has better objective,high accurate of diagnosis,well repeats as well.It deserves to be used.