Correlation between Expression of P38 MAPK-Signaling and uPA in Breast Cancer |
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作者单位: | Yanchun Han1 Luying Liu1 Dongxia Yan1 Guihua Wang2 1Department of Pathology;2Department of Physiology,Binzhou Medical University,Bin-zhou 256603,Shandong Province,China. |
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摘 要: | OBJECTIVE To study the expression of phosphorylated p38 mitogen-activated protein kinase(p-p38)and uPA and the correlation of their expression with breast cancer clinicopathological characteristics,and to investigate the role of the p38MAPK-signaling pathway in regulating uPA expression in breast cancer cells. METHODS Immunohistochemistry(S-P) was used to test the expression of p-p38 and uPA in 60 specimens of breast cancer tissues.Western blots were adopted to detect expression of the p-p38 and uPA proteins in MDA-MB-231 and MCF-7 breast cancer cells,and uPA expression a er treatment with SB203580,a specific inhibitor of p38 MAPK. RESULTS The positive rate of the p-p38 protein and uPA protein expression in the breast cancer tissues was 56.7% and 60.0%,respectively.The expression of p-p38 was positively related to the expression of uPA(r=0.316,P<0.05).The expression of p-p38 and uPA was related to lymph node metastas is and the TNM stage(P<0.05),but it was not related to the patient’s age or tumor size(P>0.05).The expression of p-p38 and uPA in MDA-MB-231 cells was higher than that in MCF-7 cells.SB203580 inhibited the p38 MAPK pathway and reduced uPA protein expression. CONCLUSION The p38 MAPK-signaling pathway promotes breast cancer malignant progression by up-regulating uPA expression,and it may be an important process in breast cancer invasion and metastasis.
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关 键 词: | 乳腺癌 P38 MAPK uPA 基因表达 肿瘤标志物 |
收稿时间: | 14 March 2007 |
Correlation between expression of P38 MAPK-signaling and uPA in breast cancer |
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Authors: | Yanchun Han Luying Liu Dongxia Yan Guihua Wang |
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Institution: | 1. Department of Pathology, Binzhou Medical University, Binzhou 256603, Shandong Province, China 2. Department of Physiology, Binzhou Medical University, Binzhou 256603, Shandong Province, China |
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Abstract: | OBJECTIVE To study the expression of phosphorylated p38 mitogen.Activated protein kinase(p-p38)and uPA and the correlation of their expression with breast cancer Clinic.patholodiCal characteristics,and to investigate the role of the p38MAPK-signaling pathway in regulating uPA expression in breast cancer cells.METHODS Immunohistochemistry(S-P)was used to test the expression of P-p38 and uPA in 60 specimens of breast cancer tissues.Western blots were adopted to detect expression of the p-p38 and uPA proteins in MDA-MB-231 and MCF-7 breast cancer cells.And uPA expression after treatment with SB203580,a specific inhibitor of p38 MAPK.RESULTS The positive rate of the P.P38 protein and uPA protein expression in the breast cancer tissues was 56.7% and 60.0%.Respectively.The expression of P.P38 was positively related to the expression of uPA(r=0.316,P<0.05).The expression of p-p38 and uPA was related to lymph node metastasis and the TNM stage(P<0.05),but it was not related to the patient's age or tumor size(P>0.05).The expression of p-p38 and uPA in MDA. MB-231 cells was higher than that in MCF.7 cells.SB203580 inhibited the p38 MAPK pathway and reduced uPA protein expression.CONCLUSI0N The p38 MAPK-signaling pathway promotes breast cancer malignant progression by up.Regulating uPA expression,and it may be an important process in breast cancer invasion and metastasis. |
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Keywords: | p38 MAP kinase uPA breast cancer |
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