| 芍药苷的水解动力学研究 |
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| 引用本文： | 刘倩,刘永刚,李继安,喇万英.芍药苷的水解动力学研究[J].时珍国医国药,2010,21(3). |
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| 作者姓名： | 刘倩 刘永刚 李继安 喇万英 |
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| 作者单位： | 1. 华北煤炭医学院,河北,唐山,063000
2. 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 科技部国际科技合作项目 |
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| 摘 要： | 目的 考察芍药苷在不同pH条件下的水解情况. 方法Diamonsil-C_(18)色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.1%的甲酸水溶液(15∶85);检测波长为230 nm;进样量为10 μl;柱温为室温,流速为1.0 ml·min~(-1).结果 芍药苷对照品在0.021 5～0.215 μg范围内,进样量与峰面积间呈良好的线性关系,r=0.999 9,并得到不同pH条件下的水解动力方程.结论 芍药苷的水解符合一级动力学模型.在不同pH条件下,芍药苷的水解速度不同.
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| 关 键 词： | 芍药苷 水解动力学 高效液相色谱 |
Study on Hydrolysis Kinetics of Paeoniflorin |
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| LIU Qian,LIU Yong-gang,LI Jian,LA Wan-ying.Study on Hydrolysis Kinetics of Paeoniflorin[J].Lishizhen Medicine and Materia Medica Research,2010,21(3). |
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| Authors: | LIU Qian LIU Yong-gang LI Jian LA Wan-ying |
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| Institution: | LIU Qian1,LIU Yong-gang2,LI Ji-an1,LA Wan-ying1(1.North China Coal Medical University,Tangshan 063000,China,2.College of Traditional Chinese Pharmacology,Beijing University of Traditional Chinese Medicine,Beijing 100102,China) |
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| Abstract: | Objective To investigate the hydrolysis kinetics of Paeoniflorin in different pH values.Methods Diamonsil-C_(18) column(250mm×4.6mm,5μm) was used with the use of the acetonitriler-0.1%formic acid (15∶85)as the mobile phase, the flow rate was 1.0 ml·min~(-1) and the detection wavelength was set at 230 nm.Results Paeoniflorin was well separated,the linear range of paeoniflorin was 0.021 5～0.215 μg (r=0.999 9). Hydrolysis kinetics equations in different pH values was obtained.Conclusion The degradation follows pseudofirst-order kinetics. Hydrolysis rate is different in various pH values. |
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| Keywords: | Paeoniflorin Hydrolysis Kinetics HPLC |
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