| Effects of Triptolide on Cell Proliferation and CXCR4 Expression in Burkitt＇s Lymphoma Raji Cells In Vitro |
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| 引用本文： | 张纯,崔国惠,刘芳,吴秋玲,陈燕. Effects of Triptolide on Cell Proliferation and CXCR4 Expression in Burkitt＇s Lymphoma Raji Cells In Vitro[J]. 中国癌症研究, 2007, 19(1): 27-31. DOI: 10.1007/s11670-007-0027-3 |
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| 作者姓名： | 张纯 崔国惠 刘芳 吴秋玲 陈燕 |
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| 作者单位： | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 |
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| 摘 要： | Objective： To investigate the inhibitory effects of triptolide on cell proliferation and CXCR4 expression in Burkitt＇s lymphoma cell line Raji cells. Methods： The effects of triptolide on the growth of Raji cells were studied by 3-（4, 5-Dimethyl-2-thiazolyl）-2, 5-diphenyl-2H-tetrazolium（MTT） assay. The effects of triptolide on CXCR4 expression on Raji cells were studied by flow cytometric analysis. Chemotaxis assays were performed to observe the effects of triptolide on migration of Raji cells towards recombinant human SDF-1α （rhSDF-1α） in vitro. Results： Triptolide inhibited the proliferation of Raji cells in a dose- and time-dependent way with a 24-h IC50 value of 43.06 nmol/L and a 36-h IC50 value of 25.08 nmol/L. Triptolide could downregulate the CXCR4 expression on Raji cells in a dose-dependent manner. Furthermore, chemotaxis assays showed that triptolide could block the migration of Raji cells to rhSDF-1α in vitro, and the inhibition was dose-dependent. Conclusion： Triptolide could inhibit the proliferation and migration of Raji cells in vitro. The underlying anti-tumor mechanism of triptolide might be related to the anti-proliferative effect and the blockage of SDF-1/CXCR4 axis.
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| 关 键 词： | 淋巴瘤 Raji细胞 雷公藤内酯醇 细胞增殖 CXCR4 基因表达 |
| 文章编号： | 1000-9604（2007）01-0027-05 |
| 收稿时间： | 2006-10-23 |
| 修稿时间： | 2006-12-28 |
Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro |
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| Zhang Chun,Cui Guo-hui,Liu Fang,Wu Qiu-ling,Chen Yan. Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro[J]. Chinese Journal of Cancer Research, 2007, 19(1): 27-31. DOI: 10.1007/s11670-007-0027-3 |
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| Authors: | Zhang Chun Cui Guo-hui Liu Fang Wu Qiu-ling Chen Yan |
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| Affiliation: | (1) Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China |
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| Abstract: | Objective To investigate the inhibitory effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma cell line Raji cells. Methods The effects of triptolide on the growth of Raji cells were studied by 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium(MTT) assay. The effects of triptolide on CXCR4 expression on Raji cells were studied by flow cytometric analysis. Chemotaxis assays were performed to observe the effects of triptolide on migration of Raji cells towards recombinant human SDF-1α (rhSDF-1α) in vitro. Results Triptolide inhibited the proliferation of Raji cells in a dose-and time-dependent way with a 24-h IC50 value of 43.06 nmol/L and a 36-h IC50 value of 25.08 nmol/L. Triptolide could downregulate the CXCR4 expression on Raji cells in a dose-dependent manner. Furthermore, chemotaxis assays showed that triptolide could block the migration of Raji cells to rhSDF-1α in vitro, and the inhibition was dose-dependent. Conclusion Triptolide could inhibit the proliferation and migration of Raji cells in vitro. The underlying anti-tumor mechanism of triptolide might be related to the anti-proliferative effect and the blockage of SDF-1/CXCR4 axis. This project was supported by the National Natural Science Foundation of China (No. 30472267). |
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| Keywords: | Triptolide Raji cells Proliferation CXCR4 |
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