IGFBP-2mRNA表达在胃癌增殖分化及腹膜转移中的作用

目的　探讨胰岛素样生长因子结合蛋白 2 (IGFBP- 2 ) m RNA在胃癌组织中的表达及其在胃癌发生、腹膜转移中的作用。方法　术中收集 35例发生腹膜转移的胃癌患者的正常粘膜、原发灶、腹膜转移灶标本 ,用实时定量 PCR方法测定其 IGFBP- 2 m RNA表达 ,同时行病理切片 HE染色。结果　 IGFBP- 2在原发灶中表达与正常粘膜、腹膜转移灶相比均明显增高 (P<0 .0 5 ) ;IGFBP- 2表达在正常粘膜与腹膜转移灶间差异无显著性 (P>0 .0 5 )。结论　 IGFBP- 2在胃癌的增殖分化、腹膜转移中可能发挥着一定作用 ,可为临床预测与治疗胃癌腹膜转移提供新的方法。

IGFBP-2 mRNA Expression in Gastric Cancer Tissues and Its Roles in Proliferation,Differentiation and Peritoneal Metastasis of Gastric Cancer

Objective To detect the expression of IGFBP-2 mRNA in gastric cancer tissues and its roles in neoplasia and peritoneal metastasis of gastric cancer. Methods The IGFBP-2 mRNA expression levels in the fresh tissues from normal mucosa, primary cancer and peritoneal metastasis of 35 patients with gastric cancer were determined by the quantitative real time RT-PCR method, and the samples were sliced up and stained with hematoxylin-eosin for pathologyical examination. Results The levels of IGFBP-2 mRNA expression in gastric primary cancer tissues were significantly higher than those in the tissues from normal mucosa and peritoneal metastasis (P<0.05). Yet, IGFBP-2 mRNA expression levels showed no difference between the tissues of normal mucosa and peritoneal metastasis (P>0.05). Conclusion The expression of IGFBP-2 mRNA possibly plays an important role in proliferation, differentiation and peritoneal metastasis of gastric cancer, and it might be useful in a new method for clinical prognostication and treatment of gastric cancer peritoneal metastasis.