| 单叶蔓荆种质资源遗传多态性的RAPD分析 |
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| 引用本文： | 孙维洋,李剑芳,邱进,徐凌川.单叶蔓荆种质资源遗传多态性的RAPD分析[J].中国医药导报,2011,8(33):5-7. |
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| 作者姓名： | 孙维洋 李剑芳 邱进 徐凌川 |
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| 作者单位： | 山东中医药大学药学院,山东济南,250355 |
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| 摘 要： | 目的：利用RAPD技术分析野生单叶蔓荆（Vitex trifolia L.var.simplicifolia Cham.）种质资源的遗传多态性及种内遗传差异。方法：本研究以7个不同产区的野生蔓荆为试材,采用RAPD技术对其遗传物质进行分析,结合NTSYS-2.10e软件进行遗传相似系数计算和UPGMA方法聚类分析。结果：10个随机引物共获得88条DNA谱带,其中46条（52.3%）具有多态性;采用CTAB法提取DNA时,先将试材在0.9%的蔗糖溶液中暗培养48 h,有利于提高DNA提取率和纯度。结论：各种质之间均存在一定的遗传差异,与地域关系和主要有效成分的化学差异并不一致,尚需进一步的植物逆境生理和功能基因组学研究来解释这一现象。
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| 关 键 词： | 单叶蔓荆 遗传多样性 随机扩增多态性 |
Analysis on genetic polymorphism in germplasm materials of Vitex trifolia L.var.simplicifolia Cham.by RAPD |
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| SUN Weiyang,LI Jianfang,QIU Jin,XU Lingchuan.Analysis on genetic polymorphism in germplasm materials of Vitex trifolia L.var.simplicifolia Cham.by RAPD[J].China Medical Herald,2011,8(33):5-7. |
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| Authors: | SUN Weiyang LI Jianfang QIU Jin XU Lingchuan |
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| Institution: | SUN Weiyang,LI Jianfang,QIU Jin,XU Lingchuan School of Pharmacy,Shandong University of TCM,Shandong Province,Ji'nan 250355,China |
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| Abstract: | Objective: To study genetic polymorphism in germplasm materials of wild Vitex trifolia L.var.simplicifolia Cham.and species differences of the genetic by RAPD.Methods: 7 wild germplasms of Vitex trifolia L.var.simplicifolia Cham.from different areaes were analyzed in this study.RAPD was used to analyze the genetic material,and the NTSYS-2.10e software was used to calculate the genetic similarity coefficient,the UPGMA method was used to do the clustering analysis.Results: 88 DNA bands were amplified with 10 ... |
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| Keywords: | Vitex trifolia L var simplicifolia Cham Genetic polymorphism RAPD |
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