高效液相色谱法测定苯妥英钠血药浓度

目的：建立测定血清中苯妥英钠浓度的高效液相色谱法。方法：以HypersilC。8柱（4．6mm×200mm，10μm）为色谱柱，流动相为甲醇一水（50：50），流速为0．8ml／min，检测波长为230nm，血清样品加入3μl硫酸溶液（6M）混匀后用重蒸乙醚提取。结果：苯妥英钠血清样品线性范围为2．00-150．00mg／L，低、中、高三种不同浓度（8．33、33．33、100．00mg／L）加样回收率分别为98．40％、95．50％、102．71％，日内、日间RSD均小于5％。结论：本方法准确性好，灵敏度高，操作简便，可满足苯妥英钠血药浓度监测要求。

Determination on the concentration of Phenytoin Sodium in serum by HPLC

Objective： To establish HPLC method to determine the concentration of Phenytoin Sodium in serum. Methods： The chromatographic column was Hypersil C18 （4.6 mm×200 mm, 10 μm）, the mobile phase was methanol and water （50：50）, the flow rate was 0.8 ml/min, and the detective wavelength was 230 nm. 3 Ixl sulphuric acid solution （6 M） was added to serum and was mixed evenly, then Phenytoin Sodium was extracted by evaporated ethylether. Results： The linear range of Phenytoin Sodium in serum was 2.00-150.00 mg/L. The recovery rates of mild, middle and high concentration （8.33, 33.33, 100.00 mg/L） were 98.40%, 95.50%, 102.71% respectively, and the within-day RSD and day to day RSD were both lower than 5%. Conclusion： The method is accurate, sensitive and simple, which can satisfy the requisition of determination of Phenytoin Sodium in serum.