八角茴香油的提取工艺及抗菌、抗氧化性作用

目的：研究八角茴香油的提取工艺、抗菌、抗氧化作用。方法：采用95％乙醇做溶剂，通过正交试验、体外抑菌试验、对DPPH一自由基清除能力试验分别对八角中的八角茴香油的提取工艺、抑菌作用、自由基清除能力等进行研究。结果：试验结果表明，八角茴香与95％乙醇溶液比为1：6，虹吸次数为6次，浸泡时间为2．4h是八角茴香油的较佳提取工艺。八角茴香油浓度在15．62-62．50mg／L时对大肠埃希菌、金黄色葡萄球菌、酵母菌及黑曲霉菌等呈中度敏感：对DPPH-自由基的清除能力随着浓度的增加而增强。结论：采用乙醇回流提取八角茴香油工艺的较佳条件为A2B2C3，八角茴香油对细菌、真菌均有一定的抗菌作用和抗氧化性。

Extraction process of star anise oil and its antibacterial and antioxidative effects

Objective： To study the extraction process of star anise oil and its antibacterial and antioxidative effects. Methods： The extraction process, antibacterial effects and radicals scavenging ability of star anise oil in star anise were studied by the orthogonal test, in vitro antibacterial test and para-DPPH-radical scavenging ability test using 95% ethanol as the solvent. Results： The results showed that the best star anise oil extraction process was star anise to 95% ethanol solu- tion ratio of 1：6, siphon times of 6 times and soaking time of 2A hours. Star anise oil at the concentration of 15.62 to 62.50 mg/ml was moderately sensitive to escherichia coli, staphylococcus aureus, yeasts, aspergillus niger and others, and its para- DPPI-I-radical scavenging ability increased with the increasing concentration. Conclusion： The best conditions of star anise oil extraction process using the ethanol reflux are A2B2C3. Star anise oil has certain antibacterial and antioxidative ef- fects on bacteria and fungi.