非水毛细管电泳内标法测定麻黄中麻黄碱的含量

目的：以品红为内标,建立非水毛细管电泳（NACE）内标法测定麻黄中麻黄碱的含量。方法：采用毛细管区带电泳分离模式,以30 mmol/L的醋酸铵和醋酸钠甲醇溶液为运行缓冲液,品红为内标物,操作电压20 kV,电迁移进样10 kV×10 s,检测波长为210 nm。结果：麻黄碱在3.125～200.000 mg/L（相关系数r=0.994 0）范围内有良好的线性关系,最低检测限为2.0 mg/L,仪器精密度为2.05%,方法精密度为3.17%,回收率为97.47%～101.85%。结论：此方法为麻黄碱的测定提供了一种简便、快速、定量可靠的方法,可用于医学研究样品和药材中麻黄碱的测定。

Determination of ephedrine in ephedras by nonaqueous capillary elec-trophoresis by internal standard method

Objective： To establish nonaqueous capillary electrophoresis（NACE） method for the determination of ephedrine in ephedras by internal standard with fuchsin was the internal standard sample.Methods： Based on the mode of capillary zone electrophoresis（CZE） electrophoretic separation,30 mmol/L ammonium acetate and sodium acetate as the running buffer and fuchsin as the internal standard sample were used,with a constant voltage was 20 kV,electromigration injection was 10 kV×10 s and detection wavelength was 210 nm.Results： Linearity was obtained in the range of 3.125-200.000 mg/L for ephedrine（r=0.994 0）.The LLOQ was 2.0 mg/L,instrument precision was 2.05%,method precision was 3.17%,and the recovery rate was 97.47%-101.85%.Conclusion： This method,with an adequate internal standard and non-aqueous electrophoresis medium,optimized equipment parameters and simplified pretreatment procedure,is convenient,rapid,quantitative and reliable for determining ephedrine,and also can be used for the determination of ephedrine in medical samples and herbs.