| 乌头多糖的提取分离与含量测定 |
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| 引用本文： | 叶思勇,邵孟霞,陈智. 乌头多糖的提取分离与含量测定[J]. 中国医药导报, 2011, 8(33): 70-71 |
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| 作者姓名： | 叶思勇 邵孟霞 陈智 |
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| 作者单位： | 山东省济南市第二人民医院药剂科,山东济南,250001 |
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| 摘 要： | 目的：从中药乌头（Aconitum Paniculigerum Nakai）中提取多糖并对其进行含量测定。方法：乙醇超声提取得到多糖,采用蒽酮-硫酸比色法、紫外可见分光光度法对其进行含量测定,检测波长为（490±1）nm。结果：乌头多糖在0.020 2～0.101 0 mg/ml范围内有良好的线性关系,r=0.999 7,平均回收率为100.60%,RSD=1.97%。药材中多糖以葡萄糖为标准含量,含量为15.82%。结论：该方法准确快速、重现性好。
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| 关 键 词： | 乌头 多糖 提取 蒽酮-硫酸法 |
Studies on extraction and determination of polysaccharide in Aconitum Paniculigerum Nakai |
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| YE Siyong,SHAO Mengxia,CHEN Zhi. Studies on extraction and determination of polysaccharide in Aconitum Paniculigerum Nakai[J]. China Medical Herald, 2011, 8(33): 70-71 |
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| Authors: | YE Siyong SHAO Mengxia CHEN Zhi |
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| Affiliation: | YE Siyong,SHAO Mengxia,CHEN Zhi Department of Pharmacy,the Second People's Hospital of Ji'nan City,Shandong Province,Ji'nan 250001,China |
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| Abstract: | Objective： To extract and determine the content of polysaccharide in Aconitum Paniculigerum Nakai.Methods： Polysaccharide was abtained by ethanol,and the content of polysaccharide was identified by anthrone-sulfuricacid colorimetry and UV method with the wavelength was（490±1） nm.Results： Polysaccharide had a good linearity within 0.020 2-0.101 0 mg/ml（r=0.999 7）,the average recovery was 100.60% and RSD was1.97%.Conclusion： This method is quick,accurate and reliable for determination of Aconitum Paniculigerum Nakai. |
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| Keywords: | Aconitum Paniculigerum Nakai Polysaccharide Extraction Anthrone-sulfuricacid determination |
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