发酵虫草菌粉对细胞色素P450酶的影响研究

目的:研究发酵虫草菌粉对大鼠细胞色素P450酶CYP1A2,CYP3A4,CYP2E1的影响.方法:用Cocktail探针药物法,将Wistar大鼠按体重随机分2组,灌胃给予33 g·L~(-1)的虫草菌粉生理盐水混悬液给药组,生理盐水对照组,灌胃体积为8.5 mL·kg~(-1)诱导10 d后静脉注射给予3种探针药物咖啡因、氨苯砜、氯唑沙宗,股动脉插管取血浆,通过HPLC检测各探针药物的代谢以评价各组CYP1A2,CYP3A4,CYP2E1业邱酶的活性;药动学计算采用DAs 2.0软件完成.结果:给予发酵虫草菌粉组的大鼠,咖啡因、氨苯砜代谢均加快,t_(1/2)分别为(2.90±0.47)h,(4.83±0.60)h;氯唑沙宗t_(1/2)与对照组无显著差异.结论:发酵虫草菌粉对大鼠CYP1A2,CYP3A4有诱导作用,对CYP2E1的作用不明显.

Experimental study on cytochrome P450 enzymes after receiving ferment powder caterpillar fungus

Objective: To study the effect of ferment powder caterpillar fungus on cytochrome P450 isozymes CYP1A2, CYP3A4 and CYP2E1. Method: The methods of Cocktail probe drugs were used. The rats were randomly divided into two groups. One group were given ferment powder caterpillar fungus once daily orally for ten days. Another group received orally normal saline one daily as the blank control. After ten days of treatment, the rats were given probe drugs of coffine, dapsone and chlorzoxazone and the blood was taken out by femoral catheterization. The plasma concentration of probe drugs were determined by HPLC. Data of plasma drug level-time were disposed with DAS Ver 2.0. Result: The metabolism of caffeine and dapsone speeded up after receiving ferment powder caterpillar fungus, but the metabolism of chlorzoxazone was hardly changed. Conclusion: It suggested that ferment powder caterpillar fungus tended to be the inducer of CYP1A2 and CYP3A4. But the CYP2E1 was hardly affected.