超声声致孔隙效应增强兔前列腺组织通透性的研究

目的 探讨超声联合造影剂微泡的超声声致孔隙效应开放血-前列腺屏障,提高前列腺组织通透性的可行性.方法 15只健康8月龄性成熟新西兰兔,随机分为单纯微泡组、单纯超声组、超声联合微泡辐照组,超声直接辐照前列腺,荧光显微镜、体视显微镜、伊文思蓝(EB)示踪观察前列腺组织通透性的变化.结果 体视显微镜下超声联合微泡辐照组前列腺组织可见蓝色EB分布于前列腺包膜,部分EB渗透到腺体实质,腺体呈均匀蓝染,单纯超声组及单纯微泡组EB均分布于包膜,腺体实质部分渗透不明显.单纯超声组、单纯微泡组、超声联合微泡辐照组前列腺冰冻切片均可见红色EB荧光分布;经苏木素复染后可见超声联合微泡辐照组腺体管腔内有EB荧光,而单纯超声组、单纯微泡组均未见管腔内红色EB荧光;超声联合微泡辐照组前列腺EB平均浓度较单纯超声组、单纯微泡组高,差异有统计学意义(P<0.01),单纯微泡组及单纯超声组EB浓度均值未见显著差异(P>0.05).结论 超声联合造影剂微泡的超声声致孔隙效应可以有效开放血-前列腺屏障,明显提高兔前列腺组织通透性.

Impact of prostate permeability by sonoporation:an preliminary study

Objective To prove the feasibility of opening up the blood-prostate barrier by microbubble mediated sonoporation.Methods Fifteen male New Zealand white rabbits were randomly divided into 3 groups:ultrasound(US)group,microbubble(MB)group,ultrasound and microbubble(US+MB)group.Ultrasound was insonated directly on the prostate.Fluorescence microscopy,stereoscopic microscopy and Evan's Blue(EB)extraction were applied to trace the changes of the permeability of the blood-prostate barrier under different conditions.Results The staining of EB could be found in the surrounding or central area Of the prostates in US+MB group,but EB stained only the capsule area in US group or MB group,not the gland parenchyma.Red fluorescence of EB was found in all of the groups.After stained with haematoxylin,red fluorescence remained both in fibromuscular tissues and gland cavities in the US+MB group,but not in the US group and the MB group.The average concentration of EB in the US+MB group(10.385±0.790)μg/g]was significantly higher(P<0.01)than that of the US group(6.706±0.312)μg/g]and the MB group(6.258±0.352)μg/g].There were no significant difference between US group and MB group in EB average concentration(P>0.05).Conclusions Microbubble mediated sonoporation can open up the blood-prostate barrier with an increased permeability of the prostate.