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HBV感染人胎盘滋养层细胞机制的初步探讨
引用本文:王安辉,汪爱勤,徐德忠,门可,闫永平,张景霞,刘媛,黄晓峰,王春梅.HBV感染人胎盘滋养层细胞机制的初步探讨[J].中华实验和临床病毒学杂志,2008,22(1):51-53.
作者姓名:王安辉  汪爱勤  徐德忠  门可  闫永平  张景霞  刘媛  黄晓峰  王春梅
作者单位:1. 第四军医大学流行病学教研室,西安,710061
2. 西安通信学院门诊部
3. 第四军医大学电镜中心,西安,710061
基金项目:国家自然科学基金,解放军医药卫生科研项目 
摘    要:目的 采用透射电镜观察HBV体外感染人胎盘滋养层细胞的超微结构变化.方法 HBV体外感染人胎盘滋养层细胞.ELISA检测培养上清中HBsAg,PCR检测细胞培养上清和滋养层细胞中的HBV DNA.HBV荧光定量PCR检测细胞培养上清中HBV DNA量(拷贝/ml).透射电镜观察滋养层细胞的超微结构.结果 感染组滋养层细胞培养上清中HBsAg在PBS清洗后12 h时A值为0.942,96 h时上升为1.264.PCR检测感染组细胞培养上清和感染组细胞HBV DNA均为阳性.PBS彻底清洗后0、12、36、60、84 h感染组细胞培养上清的HBV DNA分别为:<103,3×104,6×105,5×105,3×105拷贝/ml.透射电镜观察到感染组滋养层细胞膜附近存在包涵素(Clathrin)形式的内吞小体形成,并且发现内吞小体内存在病毒颗粒样结构.在感染组细胞粗面内质网内发现HBsAg特异性的纤维丝状结构.结论 HBV可能经包涵素依赖的细胞内吞形式进入滋养层细胞,进而实现感染细胞或通过胞释作用将病毒排至细胞的对侧而实现穿越滋养层细胞屏障.

关 键 词:肝炎病毒  乙型  感染  胞吞作用

The mechanism of HBV infection of human trophoblast cell
WANG An-hui,WANG Ai-qin,XU De-zhong,MEN Ke,YAN Yong-ping,ZHANG Jing-xia,LIU Yuan,HUANG Xiao-feng,WANG Chun-mei.The mechanism of HBV infection of human trophoblast cell[J].Chinese Journal of Experimental and Clinical Virology,2008,22(1):51-53.
Authors:WANG An-hui  WANG Ai-qin  XU De-zhong  MEN Ke  YAN Yong-ping  ZHANG Jing-xia  LIU Yuan  HUANG Xiao-feng  WANG Chun-mei
Institution:Department of Epidemiology, School of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China. wanganhui@hotmail.com
Abstract:OBJECTIVE: To observe the changes of human trophoblast cells after infected with hepatitis B virus. METHODS: HBV positive serum was used to infect human trophoblast cells in vitro. HBsAg in cell culture medium were detected by ELISA method and HBV DNA in cell culture medium and cells were detected by PCR method. HBV fluorescence polymerase chain reaction diagnose kit were used to detect the HBV DNA concentration. Ultra structure of trophoblast cells were observed with transmission electron microscopy (TEM). RESULTS: HBsAg could be detected in infection group by ELISA. Infection group cell culture medium and infection group cells were HBV DNA positive. HBV DNA concentrations in HBV infection cell culture medium in 0, 12, 36, 60, 84 h after extensively PBS washed were < 10(3), 3 x 10(4), 6 x 10(5), 5 x 10(5), 3 x 10(5) copies/mL. HBV infected trophoblast cells were found many forms of endosomes, some of which contents virus like particle. CONCLUSION: HBV might take advantage of clathrin-mediated endocytosis to enter trophoblast cell, which might lead to cell infection or across the cell bar by transcytosis.
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