孟鲁司特与桂龙咳喘宁对哮喘模型血清内皮素与一氧化氮含量的影响

目的研究孟鲁司特与桂龙咳喘宁对支气管哮喘小鼠模型血清内皮素-1（ET-1）与一氧化氮（NO）含量的影响。方法将30只实验小鼠随机分为正常组、哮喘模型组、孟鲁司特组、桂龙咳喘宁组及联合用药组，每组6只。以卵白蛋白致敏法制作小鼠哮喘模型，采用放射免疫分析法检测血清ET-1含量，用硝酸酶还原法检测血清NO含量。结果哮喘模型组血清中ET-1、NO的含量明显高于正常组（P〈0．05）；孟鲁司特组与联合用药组ET-1、NO含量明显低于哮喘模型组（P〈0．05），桂龙咳喘宁组ET-1、NO含量虽低于哮喘模型组，但仅ET-1比较差异有统计学意义（P〈0．05）。结论孟鲁司特与桂龙咳喘宁均能显著抑制支气管哮喘小鼠模型血清ET-1含量，两者联用可对ET-1、NO含量有显著影响。

The experimental study of bronchial bsthma therapy by using montelukast and guilongkechuanning symphysialy

Objective To observe the effects of montelukast and Guilongkechuanning on nitrogen monoxidum （NO） and endothelin （ ET-1 ） in the serum of rat with bronchial asthma. Methods 30 mice were randomly divided into 5 groups ： the nomal group, the asthma-model group, the montelukast group, the Guilongkechuanning group and the symphysial drug-using group. The bronchial asthmatic model was established by egg protein sensibilization. The content of ET-1 and NO in the serum were analyzed with nitric acid reductase methyl and RIA. Results Compared with the nomal group, the content of ET-1 and NO in the asthma-model group＇s blood serum was conspicuous increased（P 〈 0.05） ;Montelukast group and drug-using group ET- 1, NO content were significantly lower than the astham-model group （ P 〈 0.05 ） ; Although the content of ET-1 and NO were lower than the as them-model group, but only ET-1 difference was statistically significant （P 〈 0.05）. Conclusion Both mon- telukast and Guilongkechuanning can noticeably inhibition the content of ET-1. The content of ET-1 and NO in the serum of rat with bronchial asthma can be conspicuously effected by using these two drugs symphysialy.