FHIT和Caspase-3在结直肠癌组织中的表达及意义

目的探讨Caspase-3蛋白和FHIT蛋白在结直肠癌组织中的表达和意义。方法应用免疫组化S-P法检测10例正常结直肠组织，30例结直肠腺瘤、息肉组织，60例结直肠癌组织中Caspase-3蛋白和FHIT蛋白的表达。结果Caspase-3、FHIT蛋白在结直肠癌组织中的阳性表达率明显低于正常结直肠组织、结直肠腺瘤、息肉组织，差异均有统计学意义（P〈0.05和P〈0.01）。在结直肠癌组织中，随组织病理分级的增加，Caspase-3、FHIT蛋白表达阳性率逐渐下降；Caspase-3蛋白和FHIT蛋白表达呈正相关。结论FHIT、Caspase-3在结直肠癌发生、发展过程中起着重要的作用，联合检测可为结直肠癌的早期诊断、进一步治疗及预后提供必要的理论依据。

Expression and significance of FHIT and Caspase-3 protein in colorectal carcinoma

Objective To study the expression and significance of FHIT and Caspase-3 protein in colorectal carcinoma. Methods Immunohistochemical staining S-P method was used to detect the expression of FHIT protein and Caspase-3 protein in 10 cases of nomal colorectal mucosa,30 cases of colorectal adenoma and polyps and 60 cases of colorectal carcinoma. Results The positive expression rate of Caspase-3 and FHIT protein in colorectal carcinoma was lower than that in nomal colorectal mucosa and colorectal adenoma and polyps. The expression rate of Caspase-3 and FHIT protein was decreased with the increase of histological grade, and the difference has statistics significant（P〈0.05 or P〈0.01）. The expression of Caspase- 3 protein and FHIT protein was positively correlated. Conclusion FHIT and Caspase-3 may play important roles in oncogenesis and progression of colorectal carcinoma. Combined detection of Caspase-3 and FHIT protein could provide the necessary theoretical basis for the early diagnosis, further treatment and prognosis of colorectal cancer.